Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan 430056, P. R. China.
Key Laboratory of Pesticide and Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.
ACS Appl Bio Mater. 2021 Sep 20;4(9):6954-6961. doi: 10.1021/acsabm.1c00623. Epub 2021 Sep 1.
The regulation of surface wettability and cell adhesion behavior in a mild and unperturbed state at the interface remains a challenging task. To address this task, we adopt a strategy, based on bridging the host-guest recognition capacities of pillar[5]arene and good attachment for cell adhesion abilities of graphene oxide, to construct a smart pillar[5]arene triazole-linked naphthalene-modified graphene oxide interface. The hybrid surface exhibited a good stimuli-responsive selectivity toward arginine, as demonstrated by the wettability and cell adhesion behavior. Further studies at molecular levels indicated that the recognition mechanism of arginine was probably due to the formation of a host-guest complex driven by π-π stacking interactions between the cavity of pillar[5]arenes and arginine, which eventually resulted in the change in surface wettability and cellular adhesion behavior. It not only signifies a host-guest interaction strategy for the design of smart devices via the host-guest effect but also inspires the design of high-performance biointerface for affinity-adherent cells without exposing cells to harsh physical and chemical conditions.
在温和且未被干扰的界面状态下调节表面润湿性和细胞黏附行为仍然是一项具有挑战性的任务。为了解决这个问题，我们采用了一种策略，基于桥联主体-客体识别能力的杯芳烃和良好的细胞黏附能力的氧化石墨烯，构建了一种智能杯芳烃三唑键联萘基修饰的氧化石墨烯界面。该杂化表面对精氨酸表现出良好的刺激响应选择性，这可以通过润湿性和细胞黏附行为来证明。在分子水平上的进一步研究表明，精氨酸的识别机制可能是由于主客体配合物的形成，这是由杯芳烃空腔和精氨酸之间的π-π堆积相互作用驱动的，这最终导致了表面润湿性和细胞黏附行为的变化。这不仅为通过主客体效应设计智能器件提供了一种主客体相互作用策略，而且为在不暴露细胞于苛刻的物理和化学条件下的情况下设计高性能的亲和性细胞生物界面提供了启示。
