实验性葡萄膜炎中的视网膜脂质过氧化

Retinal lipid peroxidation in experimental uveitis.

作者信息

Rao N A, Fernandez M A, Cid L L, Romero J L, Sevanian A

机构信息

Department of Ophthalmology, University of Southern California, Los Angeles.

出版信息

Arch Ophthalmol. 1987 Dec;105(12):1712-6. doi: 10.1001/archopht.1987.01060120110036.

DOI:10.1001/archopht.1987.01060120110036

PMID:3500703

Abstract

Lewis rats with retinal S-antigen-induced uveitis were treated with the enzymatic antioxidants superoxide dismutase and catalase or with the hydroxyl radical scavenger dimethyl thiourea for one week. The enucleated eyes of these animals and of normal saline-treated control rats were studied for the presence of lipid peroxidation products in the retina; In addition, choroidal thickness was measured by morphometric analysis to determine the severity of choroidal inflammation. Control animals showed severe chorioretinal inflammation and presence of lipid peroxidation products. Intraocular inflammation was less severe and formation of conjugated dienes and malonaldehyde was significantly inhibited in animals treated with the enzyme antioxidants or dimethyl thiourea. These results suggest that free radicals generated during intraocular inflammation can lead to retinal damage by peroxidation of its lipid cell membranes.

摘要

用酶抗氧化剂超氧化物歧化酶和过氧化氢酶或羟基自由基清除剂二甲基硫脲对患有视网膜S抗原诱导性葡萄膜炎的Lewis大鼠进行为期一周的治疗。对这些动物以及用生理盐水处理的对照大鼠摘除的眼球进行研究，以检测视网膜中脂质过氧化产物的存在；此外，通过形态计量分析测量脉络膜厚度，以确定脉络膜炎症的严重程度。对照动物表现出严重的脉络膜视网膜炎症和脂质过氧化产物的存在。在用酶抗氧化剂或二甲基硫脲处理的动物中，眼内炎症较轻，共轭二烯和丙二醛的形成受到显著抑制。这些结果表明，眼内炎症期间产生的自由基可通过其脂质细胞膜的过氧化作用导致视网膜损伤。