Sujai Palasseri T, Shamjith Shanmughan, Joseph Manu M, Maiti Kaustabh Kumar
Chemical Sciences and Technology Division (CSTD), CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019 Kerala, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
ACS Appl Bio Mater. 2021 Jun 21;4(6):4962-4972. doi: 10.1021/acsabm.1c00241. Epub 2021 May 19.
Pancreatic cancer represents one of the most aggressive in nature with a miserable prognosis that warrants efficient diagnostic and therapeutic interventions. Herein, a MnO overlaid gold nanoparticle (AuNPs) based photothermal theranostic nanoenvelope (PTTNe:MnO@AuNPs) was fabricated to substantiate surface-enhanced Raman spectroscopy (SERS) guided real-time monitoring of photothermal therapy (PTT) in pancreatic cancer cells. A sharp enhancement of the fingerprint Raman signature of MnO at 569 cm exhibited as a marker peak for the first time to elucidate the intracellular PTT event. In this strategic design, the leftover bare AuNPs after the degradation of the MnO layer from the nanoenvelope in the presence of intracellular HO enabled real-time tracking of biomolecular changes of Raman spectral variations during PTT. Moreover, the surface of the as-synthesized nanoenvelope was functionalized with a pancreatic cancer cell targeting peptide sequence for cholecystokinin fashioned the PTTNe with admirable stability and biocompatibility. Finally, the precise cell death mechanism was explicitly assessed by SERS spectral analysis as a complementary technique. This targeted phototheranostic approach demonstrated in pancreatic cancer cells presented a therapeutically viable prototype for futuristic personalized cancer nanomedicine.
胰腺癌是最具侵袭性的癌症之一，预后很差，需要有效的诊断和治疗干预。在此，制备了一种基于MnO包覆金纳米颗粒（AuNPs）的光热诊疗纳米包膜（PTTNe:MnO@AuNPs），以证实表面增强拉曼光谱（SERS）引导下对胰腺癌细胞光热疗法（PTT）的实时监测。首次观察到MnO在569 cm处的指纹拉曼特征峰显著增强，以此作为阐明细胞内PTT事件的标记峰。在这一策略性设计中，纳米包膜中的MnO层在细胞内HO存在的情况下降解后剩余的裸AuNPs能够实时跟踪PTT过程中拉曼光谱变化的生物分子变化。此外，合成的纳米包膜表面用针对胆囊收缩素的胰腺癌细胞靶向肽序列进行功能化修饰，使PTTNe具有良好的稳定性和生物相容性。最后，通过SERS光谱分析作为一种补充技术明确评估了精确的细胞死亡机制。这种在胰腺癌细胞中展示的靶向光热诊疗方法为未来个性化癌症纳米医学提供了一种具有治疗可行性的原型。
