Zhang Sihang, Yu Sheng, Sun Jingwen, Huang Teng, Lin Hongzheng, Li Zhe, Xiao Zeyu, Lu Wei
School of Pharmacy & Minhang Hospital, Key Laboratory of Smart Drug Delivery, Ministry of Education & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.
Department of Pharmacology and Chemical Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China.
Pharmaceutics. 2024 Aug 20;16(8):1089. doi: 10.3390/pharmaceutics16081089.
Gold-based nanoparticles for surface-enhanced Raman scattering (SERS) imaging show great potential for precise tumor detection and photothermal therapy (PTT). However, the metabolizability of gold nanoparticles (Au NPs) raises big concerns. Herein, we designed a core-shelled nanostructure of copper sulfide (CuS)-coated Au NPs with surface pegylation (PEG-Au@CuS NSs). The excreted Au in the gallbladders at 1 h and 4 h in mice injected with PEG-Au@CuS NSs was 8.2- and 19.1-fold of that with the pegylated Au NPs (PEG-AuNPs) of the same Au particle size, respectively. By loading the Raman reporter 3,3'-Diethylthiatricarbocyanine iodide (DTTC) in the core-shell junction of PEG-Au@CuS NSs, the PEG-Au-DTTC@CuS NSs exhibited the Raman signal-to-noise (S/N) ratio of 4.01 after 24 h of intravenous (IV) injection in the mice bearing an orthotopic CT26-Luc colon tumor. By contrast, the DTTC-coated PEG-AuNPs (PEG-Au-DTTC NPs) achieved an S/N ratio of 2.71. Moreover, PEG-Au-DTTC@CuS NSs exhibited an increased photothermal conversion effect compared with PEG-Au-DTTC NPs excited with an 808-nm laser. PEG-Au-DTTC@CuS NSs enabled intraoperative SERS image-guided photothermal therapy for a complete cure of the colon tumor-bearing mice. Our data demonstrated that the PEG-Au-DTTC@CuS NSs are promising intraoperative Raman image-guided theranostic nanoplatform with enhanced hepatobiliary excretion.
用于表面增强拉曼散射（SERS）成像的金基纳米颗粒在精确肿瘤检测和光热疗法（PTT）方面显示出巨大潜力。然而，金纳米颗粒（Au NPs）的代谢性引发了重大担忧。在此，我们设计了一种表面聚乙二醇化的硫化铜（CuS）包覆Au NPs的核壳纳米结构（PEG-Au@CuS NSs）。在注射了PEG-Au@CuS NSs的小鼠中，1小时和4小时时胆囊中排出的Au分别是相同金粒径的聚乙二醇化Au NPs（PEG-AuNPs）的8.2倍和19.1倍。通过将拉曼报告分子3,3'-二乙基硫代三碳菁碘化物（DTTC）负载到PEG-Au@CuS NSs的核壳交界处，在原位接种CT26-Luc结肠肿瘤的小鼠静脉注射（IV）24小时后，PEG-Au-DTTC@CuS NSs的拉曼信噪比（S/N）为4.01。相比之下，DTTC包覆的PEG-AuNPs（PEG-Au-DTTC NPs）的S/N比为2.71。此外，与用808 nm激光激发的PEG-Au-DTTC NPs相比，PEG-Au-DTTC@CuS NSs表现出增强的光热转换效应。PEG-Au-DTTC@CuS NSs能够在术中进行SERS图像引导的光热疗法，以完全治愈荷结肠肿瘤小鼠。我们的数据表明，PEG-Au-DTTC@CuS NSs是一种有前景的术中拉曼图像引导的治疗诊断纳米平台，具有增强的肝胆排泄功能。
