Department of Bacteriology, University Hospital of Clermont-Ferrand, Clermont-Ferrand, France.
University Clermont Auvergne, Inserm U1071, USC-INRAE 2018, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Clermont-Ferrand, France.
Lett Appl Microbiol. 2022 May;74(5):666-670. doi: 10.1111/lam.13651. Epub 2022 Jan 25.
Tissue specimens are valuable materials for microbiological diagnosis. The method of tissue processing can have a significant effect on sensitivity. This study aimed to compare different biopsy processing methods in terms of efficacy and standardization. Pork tissue artificially inoculated with Staphylococcus aureus and Escherichia coli, and samples of infected human tissue were processed by different methods before culture, and the results compared. Bacterial recovery from artificially inoculated pork tissue was significantly higher by homogenization with GentleMacs Dissociator than with sonication. No significant difference was observed between the GentleMacs Dissociator and manual treatment with a scalpel and vortexing. The microbial yield from homogenized human tissues was significantly higher after homogenization with GentleMacs Dissociator than with the conventional method. Homogenization with the GentleMacs Dissociator retrieves bacteria from tissue effectively. Tissue homogenization with the Dissociator is easy and fast to perform and allows for a high degree of standardization.
组织标本是微生物诊断的有价值的材料。组织处理的方法会对检测的灵敏度有显著影响。本研究旨在比较不同的活检处理方法在效果和标准化方面的差异。本研究使用人工接种金黄色葡萄球菌和大肠杆菌的猪肉组织和感染人类组织的样本，在培养之前使用不同的方法进行处理，然后对结果进行比较。与超声处理相比，使用 GentleMacs 组织匀浆器进行匀浆处理可显著提高人工接种猪肉组织中细菌的回收率。GentleMacs 组织匀浆器与手术刀手动处理和涡旋处理之间没有显著差异。与传统方法相比，使用 GentleMacs 组织匀浆器进行匀浆处理可显著提高匀浆化人组织中的微生物产量。GentleMacs 组织匀浆器可有效从组织中回收细菌。使用 Dissociator 进行组织匀浆处理既简单又快速，并且可以高度标准化。
