Division of Legal Medicine, Department of Community Medicine and Social Healthcare Science, Kobe University Graduate School of Medicine, Kobe, Japan.
Forensic Science Laboratory, Hyogo Prefectural Police Headquarters, Kobe, Japan.
Leg Med (Tokyo). 2022 Mar;55:102006. doi: 10.1016/j.legalmed.2021.102006. Epub 2021 Dec 27.
Coronary artery disease (CAD), including coronary atherosclerosis (CAS), is one of the most common causes of death. The FURIN SNP rs17514846 is assumed to be a risk factor for CAD. We evaluated this relationship using autopsy specimens and autopsy data, such as the histopathological degree of CAS.
A total of 106 samples were genotyped from obtained blood samples. Myocardial and coronary arterial FURIN levels were quantified by ELISA. The degree of CAS was classified histopathologically according to the Stary classification, and the localization of FURIN was examined by immunostaining. The obtained data were analyzed statistically.
FURIN expression was widely observed in the myocardium, vascular smooth muscle cells, endothelial cells, adipocytes, and macrophages. FURIN level in the myocardium of cases with the AA genotype at the FURIN SNP rs17514846 was higher than that in CC cases. Additionally, FURIN levels in both coronary arteries and myocardium were higher at the early stage of CAS than at the late stage microscopically.
Our study suggested that the A allele of rs17514846 is associated with higher FURIN level in the heart and that FURIN exhibits a higher level in the early stage of CAS. These findings deepen our understanding of the mechanism of CAS.
冠心病（CAD），包括冠状动脉粥样硬化（CAS），是最常见的死亡原因之一。FURIN SNP rs17514846 被认为是 CAD 的危险因素。我们使用尸检标本和尸检数据（如 CAS 的组织病理学程度）评估了这种关系。
从获得的血液样本中总共对 106 个样本进行了基因分型。通过 ELISA 定量心肌和冠状动脉 FURIN 水平。根据 Stary 分类对 CAS 的程度进行组织病理学分类，并通过免疫染色检查 FURIN 的定位。对获得的数据进行统计学分析。
FURIN 在心肌、血管平滑肌细胞、内皮细胞、脂肪细胞和巨噬细胞中广泛表达。FURIN SNP rs17514846 处 AA 基因型病例的心肌 FURIN 水平高于 CC 病例。此外，CAS 早期的冠状动脉和心肌 FURIN 水平均高于显微镜下的晚期。
我们的研究表明，rs17514846 的 A 等位基因与心脏中更高的 FURIN 水平相关，并且 FURIN 在 CAS 的早期阶段表现出更高的水平。这些发现加深了我们对 CAS 机制的理解。
