Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Int J Mol Sci. 2021 Dec 24;23(1):196. doi: 10.3390/ijms23010196.
The triterpenes in bitter gourd () show a variety of medicinal activities. Oxidosqualene cyclase (OSC) plays an indispensable role in the formation of triterpene skeletons during triterpene biosynthesis. In this study, we identified nine genes encoding OSCs from bitter gourd (-). Analyses of their expression patterns in different tissues suggested that characteristic triterpenoids may be biosynthesized in different tissues and then transported. We constructed a hairy root system in which overexpression led to an increased accumulation of camaldulenic acid, enoxolone, and quinovic acid. Thus, the overexpression of increased the active components content in bitter gourd. Our data provide an important foundation for understanding the roles of McOSCs in triterpenoid synthesis.
苦瓜中的三萜类化合物具有多种药用活性。氧化鲨烯环化酶（OSC）在三萜生物合成过程中三萜骨架的形成中起着不可或缺的作用。在这项研究中，我们从苦瓜中鉴定出了 9 个编码 OSCs 的基因（-）。对它们在不同组织中的表达模式的分析表明，特征性三萜类化合物可能在不同的组织中合成，然后再运输。我们构建了一个发根系统，其中过表达导致苦瓜中羽扇豆醇二酸、烯醇酮和奎尼酸的积累增加。因此，过表达增加了苦瓜中活性成分的含量。我们的数据为理解 McOSCs 在三萜类合成中的作用提供了重要基础。
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