State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
Department of Horticulture, Faculty of Agricultural Sciences & Technology, Bahauddin Zakariya University, Multan 60000, Pakistan.
Biochem Biophys Res Commun. 2018 Nov 30;506(3):755-764. doi: 10.1016/j.bbrc.2018.04.112. Epub 2018 Apr 25.
Bok choy is an important Brassica vegetable which is also known for its wide range of cultivars that differ in their appearance, leaf color, size and shape. For the purpose to investigate the effect of these phenotypic differences on their lipid composition, seven morphotypes of NHCC (Suzhouqing, Aijaohuang, Wutacai, Yellowrose, Ziluolan, Xiangqingcai and Zicaitai) were selected for this study. For this reason, extensive metabolic approach was adopted which was mainly focused on lipidomics. The overall metabolic position of lipids was determined and the isolated lipid compounds were characterized on the basis of their lipid classes. Moreover, discriminative analysis was applied to monitor the distribution pattern of lipid in different cultivars. Aijiaohuang was the leading cultivar which contained highest lipid levels, whereas least proportion was found in Zicaitai. We proposed that leaf color might have an effect on the lipid composition such as purple cultivars were dominated in glycerophopholipids, light green in fatty acids and dark green were rich in glycerolipids. The level of metabolites differed greatly among different genotypes. Lipid-metabolite interactions revealed the positive correlation of lipids with flavonoid and hydroxycinnamoyl derivatives, whereas negative correlation was noticed in case of phenylamines. This is the first comprehensive study based on lipidomics in order to evaluate the substantial impact of various phenotypes on the metabolic composition of NHCC.
白菜是一种重要的芸薹属蔬菜，因其品种繁多而闻名，这些品种在外观、叶片颜色、大小和形状上存在差异。为了研究这些表型差异对其脂质组成的影响，本研究选择了 7 个 NHCC 形态型（苏州青、矮脚黄、乌塌菜、黄玫瑰、紫露兰、香青菜和紫彩苔）进行研究。因此，本研究采用了广泛的代谢方法，主要集中在脂质组学上。确定了脂质的整体代谢位置，并根据脂质类别对分离出的脂质化合物进行了特征描述。此外，还应用判别分析来监测不同品种中脂质的分布模式。矮脚黄是含脂量最高的主导品种，而紫彩苔的含脂量最低。我们提出，叶片颜色可能会影响脂质组成，例如，紫色品种以甘油磷酯为主，浅绿色品种以脂肪酸为主，深绿色品种则富含甘油酯。不同基因型之间的代谢物水平差异很大。脂质-代谢物相互作用表明脂质与类黄酮和羟基肉桂酰衍生物呈正相关，而与苯丙胺呈负相关。这是首次基于脂质组学对不同表型对 NHCC 代谢组成的实质性影响进行的综合评估。
