Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (Cinvestav), Mexico City 07360, Mexico.
Department of Neuroimmunology, National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNN), Mexico City 14269, Mexico.
Int J Mol Sci. 2021 Dec 28;23(1):298. doi: 10.3390/ijms23010298.
An association between varicella zoster virus (VZV) and multiple sclerosis (MS) has been reported in Mexican populations. The aim of this study was to compare the response of T cells from MS patients, during relapse and remission, to in vitro stimulation with VZV, adenovirus (AV) and Epstein-Barr virus (EBV). Proliferation and cytokine secretion of T cells from 29 relapsing-remitting MS patients and 38 healthy controls (HC) were analyzed by flow cytometry after stimulating with VZV, AV or EBV. IgG and IgM levels against VZV and EBV were quantified using Enzyme-Linked Immunosorbent Assay. Relapsing MS patients showed a higher percentage of responding CD4+ and CD8+ T cells against VZV compared to AV. In HC and remitting MS patients, proliferation of CD4+ T cells was higher when stimulated with VZV as compared to EBV. Moreover, T cells isolated from remitting patients secreted predominantly Th1 cytokines when cell cultures were stimulated with VZV. Finally, high concentration of anti-VZV IgG was found in sera from patients and controls. The results support previous studies of an VZV-MS association in the particular population studied and provide additional information about the possible role of this virus in the pathogenesis of MS.
在墨西哥人群中已经报道了水痘带状疱疹病毒（VZV）与多发性硬化症（MS）之间的关联。本研究的目的是比较来自 MS 患者在复发和缓解期的 T 细胞对 VZV、腺病毒（AV）和 EBV 的体外刺激的反应。通过流式细胞术分析 29 例复发缓解型 MS 患者和 38 例健康对照者（HC）的 T 细胞在 VZV、AV 或 EBV 刺激后的增殖和细胞因子分泌。使用酶联免疫吸附试验定量测定针对 VZV 和 EBV 的 IgG 和 IgM 水平。与 AV 相比，复发 MS 患者对 VZV 反应的 CD4+和 CD8+T 细胞的百分比更高。在 HC 和缓解 MS 患者中，与 EBV 相比，VZV 刺激的 CD4+T 细胞增殖更高。此外，当用 VZV 刺激细胞培养物时，分离自缓解患者的 T 细胞主要分泌 Th1 细胞因子。最后，在患者和对照者的血清中发现了高浓度的抗 VZV IgG。这些结果支持在研究人群中之前关于 VZV-MS 关联的研究，并提供了有关该病毒在 MS 发病机制中可能作用的更多信息。
