Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei 10048, Taiwan.
Department of Urology, National Taiwan University Hospital, Taipei 10002, Taiwan.
Int J Mol Sci. 2022 Jan 1;23(1):487. doi: 10.3390/ijms23010487.
Bacterial sensing of environmental signals through the two-component system (TCS) plays a key role in modulating virulence. In the search for the host hormone-sensing TCS, we identified a conserved locus following , a small RNA (sRNA) gene in uropathogenic . Genes of constitute an operon, and QseF binding sites were found in the promoter region. Deletion of or resulted in reduced swarming motility and swarming-related phenotypes relative to the wild-type and the respective complemented strains. The mutant had decreased promoter activity. Both and mutants exhibited decreased promoter activity and mRNA level, while increased mRNA level was observed in both mutants. Prediction by TargetRNA2 revealed as the target of GlmY. Then, construction of the translational fusions containing various lengths of 5'UTR for reporter assay and site-directed mutagenesis were performed to investigate the -GlmY interaction in activation. Notably, loss of reduced the mRNA level, and urea could inhibit swarming in a QseF-dependent manner. Altogether, this is the first report elucidating the underlying mechanisms for modulation of swarming motility by a QseEF-regulated sRNA GlmY, involving expression of , and in uropathogenic .
细菌通过双组分系统（TCS）感应环境信号，在调节毒力方面起着关键作用。在寻找宿主激素感应 TCS 的过程中，我们在尿路致病性大肠杆菌中发现了一个保守的 基因座，它是一个小 RNA（sRNA）基因。 基因构成一个操纵子，在 启动子区域发现了 QseF 结合位点。与野生型和相应的互补菌株相比， 或 缺失导致群集运动和群集相关表型减少。 突变体的 启动子活性降低。 和 突变体的 启动子活性和 mRNA 水平均降低，而两个突变体的 mRNA 水平均升高。TargetRNA2 的预测显示 GlmY 是 的靶标。然后，构建了包含不同长度 5'UTR 的翻译融合物进行报告基因测定和定点突变，以研究 激活中 -GlmY 相互作用。值得注意的是， 的缺失降低了 的 mRNA 水平，尿素可以以 QseF 依赖的方式抑制群集运动。总之，这是首次阐明了 QseEF 调节的 sRNA GlmY 对群集运动调节的潜在机制，涉及尿路致病性大肠杆菌中 、 和 的表达。
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