Zrig Ahlem, Saleh Ahmed, Hamouda Foued, Okla Mohammad K, Al-Qahtani Wahidah H, Alwasel Yasmeen A, Al-Hashimi Abdulrahman, Hegab Momtaz Y, Hassan Abdelrahim H A, AbdElgawad Hamada
Faculty of Sciences of Gabès-City Erriadh, Zrig, Gabes 6072, Tunisia.
Department of Botany, Faculty of Science, Cairo University, Giza 12613, Egypt.
Plants (Basel). 2021 Dec 27;11(1):71. doi: 10.3390/plants11010071.
Edible sprouts are rich in flavonoids and other polyphenols, as well as proteins, minerals, and vitamins. Increasing sprout consumption necessitates improving their quality, palatability, and bioactivity. The purpose of this study was to test how KNO priming affects the sprouting process species on three species (, , and ) and their nutritional values. Targeted species of were primed with KNO, and the levels of different primary and secondary metabolites were determined. KNO induced biomass accumulation in the sprouts of the three species, accompanied by an increased content of total mineral nutrients, pigments, vitamins, and essential amino acids. Besides, our results showed that KNO enhanced the activity of nitrate reductase (NR), glutamate dehydrogenase (GDH), and glutamine synthetase (GS) enzymes, which are involved in the nitrogen metabolism and GOGAT cycle, which, in turn, increase the nitrogen and protein production. KNO treatment improved the bioactive compound activities of sprouts by increasing total phenolic and flavonoid contents and enhancing the antioxidant and antidiabetic activities. Furthermore, species-specific responses toward KNO priming were noticeable, where showed the highest antioxidant and antidiabetic activities, followed by . Overall, this study sheds the light on the physiological and biochemical bases of growth, metabolism, and tissue quality improvement impact of KNO on sprouts.
可食用芽苗富含黄酮类化合物和其他多酚类物质，以及蛋白质、矿物质和维生素。增加芽苗的消费量需要提高其品质、适口性和生物活性。本研究的目的是测试硝酸钾引发如何影响三种物种（、和）的发芽过程及其营养价值。用硝酸钾对目标物种进行引发处理，并测定不同初级和次级代谢产物的水平。硝酸钾诱导了这三种物种芽苗中的生物量积累，同时总矿质营养、色素、维生素和必需氨基酸的含量增加。此外，我们的结果表明，硝酸钾增强了参与氮代谢和谷氨酰胺合成酶循环的硝酸还原酶（NR）、谷氨酸脱氢酶（GDH）和谷氨酰胺合成酶（GS）的活性，进而增加了氮和蛋白质的产量。硝酸钾处理通过增加总酚和黄酮含量以及增强抗氧化和抗糖尿病活性，提高了芽苗的生物活性化合物活性。此外，对硝酸钾引发的物种特异性反应很明显，其中表现出最高的抗氧化和抗糖尿病活性，其次是。总体而言，本研究揭示了硝酸钾对芽苗生长、代谢和组织质量改善影响的生理和生化基础。
