Łukasiewicz Research Network-New Chemical Syntheses Institute, 24-110 Puławy, Poland.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, Poland.
Molecules. 2019 Dec 16;24(24):4608. doi: 10.3390/molecules24244608.
The aim of this research was to select parameters for supercritical extraction with CO of L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the purpose of optimization, mathematical statistics was used. Qualitative analysis of products was performed with supercritical fluid chromatography (SFC). The SFC analysis revealed a proper separation of flavonoids and phenolics acids for dedicated TFC and TPC optimal parameters. The obtained results have proved that it is a possibility to extract polar compounds with non-polar solvent under higher values of pressure and temperature and to enrich product with desired group of bioactive compounds with proper optimization. The proposed extraction technique allows to obtain on an industrial scale, using an environmentally friendly solvent, a preparation rich in biologically active nutrients that can be implemented in the cosmetics, pharmaceutical and food industries.
本研究旨在选择超临界 CO2 萃取的参数，从半工业规模下考虑，以提取作为功能性食品的 L.，以获得最高浓度的生物活性极性成分，同时保持最高的过程效率。为了优化，使用了数学统计学。采用超临界流体色谱法（SFC）对产品进行定性分析。SFC 分析表明，对于特定的 TFC 和 TPC 最佳参数，可以很好地分离黄酮类和酚酸类物质。所得结果证明，在较高的压力和温度下，用非极性溶剂提取极性化合物是可行的，并可以通过适当的优化，使产品富含所需的生物活性化合物。所提出的提取技术允许在工业规模上，使用环保溶剂，获得富含生物活性营养物质的制剂，可应用于化妆品、制药和食品工业。
