Savvidou Maria G, Ferraro Angelo, Schinas Petros, Mamma Diomi, Kekos Dimitris, Hristoforou Evangelos, Kolisis Fragiskos N
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, Greece.
Laboratory of Electronic Sensors, School of Electrical and Computer Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, Greece.
Plants (Basel). 2021 Dec 27;11(1):72. doi: 10.3390/plants11010072.
Microalgae are used in industrial and pharmaceutical applications. Their performance on biological applications may be improved by their immobilization. This study presents a way of cell immobilization using microalgae carrying magnetic properties. and cells were treated enzymatically (cellulase) and mechanically (glass beads), generating protoplasts as a means of incorporation of magnetic nanoparticles. Scanning electron microscopy images verified the successful cell wall destruction for both of the examined microalgae cells. Subsequently, protoplasts were transformed with magnetic nanoparticles by a continuous electroporation method and then cultured on a magnetic surface. Regeneration of transformed protoplasts was optimized using various organic carbon and amino acid supplements. Both protoplast preparation methods demonstrated similar efficiency. Casamino acids, as source of amino acids, were the most efficient compound for protoplasts regeneration in enzymatic and mechanical treatment, while for protoplasts regeneration, fructose, as source of organic carbon, was the most effective. Protoplasts transformation efficiency values with magnetic nanoparticles after enzymatic or mechanical treatments for and were 17.8% and 10.7%, and 18.6% and 15.7%, respectively. Finally, selected magnetic cells were immobilized and grown on a vertical magnetic surface exposed to light and without any supplement.
微藻被用于工业和制药应用中。通过固定化可以提高它们在生物应用中的性能。本研究提出了一种利用具有磁性的微藻进行细胞固定化的方法。对细胞进行酶处理（纤维素酶）和机械处理（玻璃珠），产生原生质体作为掺入磁性纳米颗粒的一种手段。扫描电子显微镜图像证实了两种被检测的微藻细胞的细胞壁均被成功破坏。随后，通过连续电穿孔法用磁性纳米颗粒转化原生质体，然后在磁性表面上培养。使用各种有机碳和氨基酸补充剂优化转化原生质体的再生。两种原生质体制备方法显示出相似的效率。酪蛋白氨基酸作为氨基酸来源，是酶处理和机械处理中原生质体再生最有效的化合物，而对于原生质体再生，果糖作为有机碳来源是最有效的。酶处理或机械处理后，和的原生质体与磁性纳米颗粒的转化效率值分别为17.8%和10.7%，以及18.6%和15.7%。最后，将选定的磁性细胞固定化并在垂直磁性表面上生长，该表面暴露于光下且不添加任何物质。
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