Ye Junran, Yang Cuiqiyun, Xia Luojia, Zhu Yinjie, Liu Li, Cao Huansheng, Tao Yi
Division of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Groundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Microorganisms. 2023 Feb 20;11(2):538. doi: 10.3390/microorganisms11020538.
Single-cell sequencing (SCS) is an evolutionary technique for conducting life science research, providing the highest genome-sale throughput and single-cell resolution and unprecedented capabilities in addressing mechanistic and operational questions. Unfortunately, the current SCS pipeline cannot be directly applied to algal research as algal cells have cell walls, which makes RNA extraction hard for the current SCS platforms. Fortunately, effective methods are available for producing algal protoplasts (cells without cell walls), which can be directly fed into current SCS pipelines. In this review, we first summarize the cell wall structure and chemical composition of algal cell walls, particularly in Chlorophyta, then summarize the advances made in preparing algal protoplasts using physical, chemical, and biological methods, followed by specific cases of algal protoplast production in some commonly used eukaryotic algae. This review provides a timely primer to those interested in applying SCS in eukaryotic algal research.
单细胞测序（SCS）是一种用于进行生命科学研究的革命性技术，它提供了最高的基因组规模通量和单细胞分辨率，并在解决机制和操作问题方面具有前所未有的能力。不幸的是，目前的SCS流程不能直接应用于藻类研究，因为藻类细胞有细胞壁，这使得当前的SCS平台难以进行RNA提取。幸运的是，有有效的方法可用于制备藻类原生质体（无细胞壁的细胞），这些原生质体可以直接用于当前的SCS流程。在本综述中，我们首先总结藻类细胞壁的结构和化学成分，特别是绿藻门的，然后总结使用物理、化学和生物学方法制备藻类原生质体所取得的进展，接着介绍一些常用真核藻类中藻类原生质体生产的具体案例。本综述为那些有兴趣将SCS应用于真核藻类研究的人提供了及时的入门指南。
