Rollet C, Jardillier J C
Ann Biol Clin (Paris). 1977;35(5):367-77.
The study of isoenzymes is often of great interest in clinical biology however, as far as alkaline phosphatase is concerned, no simple method is available to evaluate the forms of bony, intestinal, hepatic or placental origin. In most cases, it is necessary to combine several technic: electrophoresis, denaturation by heat, urea or phenyl alanine. This already complex analytical problem is rendered still more difficult by the choice of reference isoenzyme to be used calibration of the technic. The interest of this determination, especially in the field of oncology and pediatrics, should however encourage the biologist to pursue this route.
同工酶的研究在临床生物学中常常备受关注。然而，就碱性磷酸酶而言，目前尚无简单方法可用于评估骨源性、肠源性、肝源性或胎盘源性的同工酶形式。在大多数情况下，有必要结合多种技术：电泳、热变性、尿素变性或苯丙氨酸变性。由于要选择用于技术校准的参考同工酶，这个原本就复杂的分析问题变得更加困难。然而，这种测定的重要性，尤其是在肿瘤学和儿科学领域，应该会鼓励生物学家继续探索这条道路。
