[Kinetic method for determination of isoenzymes of alkaline phosphatase in human blood serum].

Resistance of liver, bone, intestinal and placental human alkaline phosphatase isozymes to the effect of L-phenylalanine and urea was studied. The combined treatment with urea and L-phenylalanine increased an accuracy of estimation due to inhibition of bone and intestinal isozymes. A special equation enabled to simplify the calculations. As shown by laboratory and clinical tests, the method developed is suitable for quantitative estimation of alkaline phosphatase isoenzymes.