Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, 60131 Ancona, Italy.
School of Biological Sciences, Institute for Global Food Security, Queens University Belfast, Belfast BT9 5DL, UK.
Int J Environ Res Public Health. 2022 Jan 5;19(1):574. doi: 10.3390/ijerph19010574.
Exposure to Endocrine Disrupting Chemicals (EDC) has been linked with several adverse outcomes. In this review, we examine EDCs that are pervasive in the environment and are of concern in the context of human, animal, and environmental health. We explore the consequences of EDC exposure on aquatic life, terrestrial animals, and humans. We focus on the exploitation of genomics technologies and in particular whole transcriptome sequencing. Genome-wide analyses using RNAseq provides snap shots of cellular, tissue and whole organism transcriptomes under normal physiological and EDC perturbed conditions. A global view of gene expression provides highly valuable information as it uncovers gene families or more specifically, pathways that are affected by EDC exposures, but also reveals those that are unaffected. Hypotheses about genes with unknown functions can also be formed by comparison of their expression levels with genes of known function. Risk assessment strategies leveraging genomic technologies and the development of toxicology databases are explored. Finally, we review how the Adverse Outcome Pathway (AOP) has exploited this high throughput data to provide a framework for toxicology studies.
接触内分泌干扰化学物质（EDC）与多种不良后果有关。在这篇综述中，我们研究了在环境中普遍存在且在人类、动物和环境健康方面令人关注的 EDC。我们探讨了 EDC 暴露对水生生物、陆地动物和人类的影响。我们专注于基因组学技术的利用，特别是全转录组测序。使用 RNAseq 进行全基因组分析可提供正常生理和 EDC 干扰条件下细胞、组织和整个生物体转录组的快照。对基因表达的全面了解提供了非常有价值的信息，因为它揭示了受 EDC 暴露影响的基因家族或更具体地说，途径，同时也揭示了不受影响的途径。通过将未知功能基因的表达水平与已知功能基因进行比较，也可以形成关于具有未知功能基因的假设。我们探讨了利用基因组学技术的风险评估策略和毒理学数据库的发展。最后，我们回顾了不良结局途径 (AOP) 如何利用这些高通量数据为毒理学研究提供框架。
