Li Bo, Huang Xiaoyu, Yang Chen, Ge Ting, Zhao Leiyun, Zhang Xiaoqiang, Tian Lintao, Zhang Enping
College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
Animals (Basel). 2021 Dec 23;12(1):28. doi: 10.3390/ani12010028.
MiRNAs are vital regulators and play a major role in cell differentiation, biological development, and disease occurrence. In recent years, many studies have found that miRNAs are involved in the proliferation and differentiation of adipocytes. The objective of this study was to evaluate the effect of miR-27a and its target gene on ovine preadipocytes differentiation in Small-tailed Han sheep (). Down-regulation of miR-27a significantly promoted the production of lipid droplets, while overexpression of miR-27a led to a reduction in lipid droplet production. In addition, inhibition of miR-27a led to a significant increase in the expression of genes involved in lipid synthesis, including PPAR γ, SCD, LPL, and FABP4. Target Scan software predicted that is a new potential target gene of miR-27a. Further experiments revealed that gene expression and protein levels were negatively correlated with miR-27a expression. Overexpression of miR-27a led to a significant decrease in mRNA levels and inhibited the accumulation of lipid droplets and vice versa. Moreover, overexpression of promoted the synthesis of lipid droplets in ovine preadipocytes. Furthermore, luciferase reporter assays confirmed to be a miR-27a direct target gene. This study confirmed that miR-27a increases the expression of genes related to lipid synthesis in ovine preadipocytes by targeting , thereby promoting the synthesis of lipid droplets. The results of this study can be used to be exploited in devising novel approaches for improving the IMF content of sheep.
微小RNA（miRNAs）是重要的调节因子，在细胞分化、生物发育和疾病发生中发挥着重要作用。近年来，许多研究发现miRNAs参与脂肪细胞的增殖和分化。本研究的目的是评估miR-27a及其靶基因对小尾寒羊绵羊前体脂肪细胞分化的影响。miR-27a的下调显著促进脂滴的产生，而miR-27a的过表达导致脂滴产生减少。此外，抑制miR-27a导致参与脂质合成的基因表达显著增加，包括过氧化物酶体增殖物激活受体γ（PPAR γ）、硬脂酰辅酶A去饱和酶（SCD）、脂蛋白脂肪酶（LPL）和脂肪酸结合蛋白4（FABP4）。Target Scan软件预测[此处原文缺失具体基因名称]是miR-27a的一个新的潜在靶基因。进一步的实验表明，[此处原文缺失具体基因名称]基因表达和蛋白水平与miR-27a表达呈负相关。miR-27a的过表达导致[此处原文缺失具体基因名称]mRNA水平显著降低，并抑制脂滴的积累，反之亦然。此外，[此处原文缺失具体基因名称]的过表达促进绵羊前体脂肪细胞中脂滴的合成。此外，荧光素酶报告基因检测证实[此处原文缺失具体基因名称]是miR-27a的直接靶基因。本研究证实，miR-27a通过靶向[此处原文缺失具体基因名称]增加绵羊前体脂肪细胞中与脂质合成相关的基因表达，从而促进脂滴的合成。本研究结果可用于设计提高绵羊肌内脂肪含量的新方法。
