The Effects of on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432.

Reducing fat deposition in sheep () tails is one of the most important ways to combat rising costs and control consumer preference. Our previous studies have shown that oar-miR-432 is differentially expressed in the tail adipose tissue of Hu (a fat-tailed sheep breed) and Tibetan (a thin-tailed sheep breed) sheep and is a key factor in the negative regulation of fat deposition through in ovine preadipocytes. This study investigated the effect of oar-miR-432 and its target genes in ovine preadipocytes. A dual luciferase assay revealed that is a direct target gene of oar-miR-432. We transfected an oar-miR-432 mimic and inhibitor into preadipocytes to analyze the expression of target genes. Overexpression of oar-miR-432 inhibits expression, whereas inhibition showed the opposite results. Compared with thin-tailed sheep, was highly expressed in the fat-tailed sheep at the mRNA and protein levels. Furthermore, we transfected the overexpression and knockdown target genes into preadipocytes to analyze their influence after inducing differentiation. Knockdown of induced ovine preadipocyte differentiation into adipocytes but suppressed oar-miR-432 expression. Conversely, the overexpression of significantly inhibited differentiation but promoted oar-miR-432 expression. overexpression also decreased the content of triglycerides. Additionally, is a nested gene in intron 1 of . When was overexpressed, the expression also increased, whereas knockdown showed the opposite results. This is the first study to reveal the biological mechanisms by which oar-miR-432 inhibits preadipocytes through and provides insight into the molecular regulatory mechanisms of in ovine preadipocytes. These results have important applications in animal breeding and obesity-related human diseases.