Konieczny Piotr, Andrzejewski Wojciech, Yang Tianyu, Urbańska Maria, Stangierski Jerzy, Tomczyk Łukasz, Mikołajczak Beata
Department of Food Quality and Safety Management, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland.
Department of Zoology, Division of Inland Fisheries and Aquaculture of Zoology, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Wojska Polskiego 71C, 60-625 Poznan, Poland.
Animals (Basel). 2021 Dec 31;12(1):90. doi: 10.3390/ani12010090.
The aim of this study was to describe the quality attributes of a freeze-dried preparation obtained from freshwater mussel (SW) soft tissue in respect to its potential as a novel pet food ingredient. After ecotoxicological testing of the raw material with MARA (Microbial Assay for Risk Assessment), the basic physico-chemical properties of the powder, such as approximate composition, bulk density, color parameters, water activity, electrophoretic analysis (SDS-PAGE), solubility, gelling and emulsifying capacity, were analyzed. The powder with a water activity of 0.43 offers a toxically safe preparation that contains over 34% protein/100 g of dry matter (DM). The SDS-PAGE profile showed twelve protein bands with a molecular weight (MW) ranging from >250 to 10 kDa. Taurine content has been estimated at an essential amount above 150 mg/100 g of DM. The powder possessed desirable emulsifying properties with 230 mL per 1 g and demonstrated the ability to form a firmer gel with a strength of 152.9 g at a temperature above 80 °C with at least 10% protein content. The L*, a*, and b* values characterizing powder color were found to be 69.49, 16.33, and 3.86, respectively. The SW mussel powder seems to be a promising ingredient that can be added with other binding or gelling agents in order to improve both the taste and acceptance of the final pet food products.
本研究的目的是描述从淡水贻贝（SW）软组织获得的冻干制剂作为新型宠物食品成分的潜在质量属性。在用MARA（风险评估微生物测定法）对原材料进行生态毒理学测试后，分析了该粉末的基本物理化学性质，如近似组成、堆积密度、颜色参数、水分活度、电泳分析（SDS-PAGE）、溶解度、凝胶化和乳化能力。水分活度为0.43的粉末提供了一种毒性安全的制剂，每100克干物质（DM）中蛋白质含量超过34%。SDS-PAGE图谱显示有12条蛋白带，分子量（MW）范围为>250至10 kDa。估计牛磺酸含量为每100克DM超过150毫克的必需量。该粉末具有理想的乳化性能，每1克为230毫升，并且在蛋白质含量至少为10%的情况下，在80℃以上的温度下能够形成强度为152.9克的更坚固凝胶。表征粉末颜色的L*、a和b值分别为69.49、16.33和3.86。SW贻贝粉似乎是一种有前景的成分，可以与其他粘合剂或胶凝剂一起添加，以改善最终宠物食品产品的口感和可接受性。
