Department of Pharmaceutical Chemistry, National University of Pharmacy of Ministry of Health of Ukraine, 4-Valentinivska St., 61168 Kharkiv, Ukraine.
Laboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukilėlių pr. 13, LT-50162 Kaunas, Lithuania.
Molecules. 2021 Dec 21;27(1):10. doi: 10.3390/molecules27010010.
L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts' biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.
藏红花（藏红花）历来被用作食品着色剂或调味剂，但最近的研究表明，它具有治疗多种与健康相关疾病的强大药理学活性。藏红花的叶子和花瓣是藏红花生产的副产品，通常不用于医药或食品工业。本研究旨在确定藏红花叶的水和乙醇提取物的化学成分，并通过 MTT 测定法测试其对黑素瘤（IGR39）和三阴性乳腺癌（MDA-MB-231）细胞系的细胞毒性活性。我们还确定了它们的抗过敏，抗炎和抗病毒活性。HPLC 指纹图谱分析表明，存在 16 种化合物，包括羟基肉桂酸，紫檀烷，类黄酮和异黄酮，它们可能有助于提取物的生物活性。首次在藏红花叶提取物中鉴定出化合物，如 tectoridin，iristectorigenin B，nigricin 和 irigenin。结果表明，芒果苷（高达 2 mg/g 干重）和异橙皮苷（8.5 mg/g 干重）是叶提取物中的主要活性成分。乙醇提取物降低了 IGR39 和 MDA-MB-231 癌细胞的活力，EC = 410±100 和 330±40μg/mL，分别。它比水提取物更活跃。槲皮素和槲皮素被鉴定为最活跃的化合物。我们的研究结果表明，藏红花叶含有具有强大细胞毒性和抗氧化活性的次生代谢产物。
