Department of Food Science, Sun Moon University, Natural Science 118, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si 336-708, Korea.
FlexPro Biotechnology, Natural Science 128, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si 336-708, Korea.
Molecules. 2021 Dec 21;27(1):21. doi: 10.3390/molecules27010021.
The present study investigated the effects of stem extract (ASE) on B16-F0 cell growth and metastasis. Evaluation of the effects of ASE on B16-F0 cells' viability and migration showed that 0.5 mg/mL ASE inhibited B16-F0 cells' growth by 30.2% and migration by 38.5%, which indicates that the ASE has anticancer and antimetastatic effects on B16-F0 cells. To study the anticancer and antimetastatic mechanism, mRNA levels of vascular endothelial growth factor (), matrix metalloproteinases-2 (), and matrix metalloproteinases-9 () expressions were evaluated with reverse transcription polymerase chain reaction, and 0.25 and 0.5 mg/mL ASE was found to exert significant inhibition on mRNA expressions of , , and in B16-F0 cells. Thus, ASE reduce extracellular matrix degradation through inhibitions of expression of and and also showed an angiogenesis inhibitory effect through reduction of expression. High-performance liquid chromatography analysis showed that among various polyphenols, gallic acid (2.1 mg/g) was a major compound of ASE. Overall, our results demonstrated that ASE inhibited the growth and migration of B16-F0 cells through downregulation of the , and genes expression, which indicates ASE could be applied for the prevention and treatment of melanoma.
本研究考察了茎提取物（ASE）对 B16-F0 细胞生长和转移的影响。评估 ASE 对 B16-F0 细胞活力和迁移的影响表明，0.5mg/mL 的 ASE 抑制了 B16-F0 细胞的生长 30.2%和迁移 38.5%，这表明 ASE 对 B16-F0 细胞具有抗癌和抗转移作用。为了研究抗癌和抗转移机制，用逆转录聚合酶链反应评估了血管内皮生长因子 ()、基质金属蛋白酶-2 () 和基质金属蛋白酶-9 () 的 mRNA 水平，发现 0.25 和 0.5mg/mL 的 ASE 对 B16-F0 细胞中 、 、 和 的 mRNA 表达有显著抑制作用。因此，ASE 通过抑制 和 的表达减少细胞外基质的降解，并且通过降低 表达也表现出抑制血管生成的作用。高效液相色谱分析表明，在各种多酚中，没食子酸（2.1mg/g）是 ASE 的主要化合物。总之，我们的结果表明，ASE 通过下调 、 和 基因的表达抑制了 B16-F0 细胞的生长和迁移，这表明 ASE 可用于预防和治疗黑色素瘤。
