Institute of Medicine, Chung Shan Medical University, 110 Sec. 1, Chien-Kuo N. Road, Taichung 40203, Taiwan.
CSMU Lung Cancer Research Center, Chung Shan Medical University, 110 Sec. 1, Chien-Kuo N. Road, Taichung 40203, Taiwan.
Molecules. 2021 Dec 23;27(1):75. doi: 10.3390/molecules27010075.
-acetylcysteine (NAC) is a recognized antioxidant in culture studies and treatments for oxidative stress-related diseases, but in some cases, NAC is a pro-oxidant. To study the effect of NAC on cell proliferation in the presence or absence of ROS stress, we used the stable ROS generator gallic acid (GA) to treat CL1-0 lung cancer cell models with different antioxidant activities. Different antioxidant activities were achieved through the ectopic expression of different single nucleotide polymorphisms. GA increased ROS levels in CL1-0/ cells and caused cell death but had no effect on CL1-0/ cells within 24 h. We found that 0.1 mM NAC eliminated GA-induced growth inhibition, but 0.5 mM NAC enhanced GA-induced CL1-0/ cell death. However, in the absence of GA, NAC exceeding 2 mM inhibited the growth of CL1-0/ cells more significantly than that of CL1-0/ cells. Without GA, NAC has an antioxidant effect. Under GA-induced ROS stress, NAC may have pro-oxidant effects. Each cell type has a unique range of ROS levels for survival. The levels of ROS in the cell determines the sensitivity of the cell to an antioxidant or pro-oxidant. Cells with different antioxidant capacities were used to show that the intracellular ROS level affects NAC function and provides valuable information for the adjuvant clinical application of NAC.
-乙酰半胱氨酸（NAC）是一种在培养研究和氧化应激相关疾病治疗中被认可的抗氧化剂，但在某些情况下，NAC 是一种促氧化剂。为了研究 NAC 在存在或不存在 ROS 应激时对细胞增殖的影响，我们使用稳定的 ROS 生成剂没食子酸（GA）处理具有不同抗氧化活性的 CL1-0 肺癌细胞模型。不同的抗氧化活性是通过异位表达不同的单核苷酸多态性来实现的。GA 增加了 CL1-0/细胞中的 ROS 水平并导致细胞死亡，但在 24 小时内对 CL1-0/细胞没有影响。我们发现 0.1 mM NAC 消除了 GA 诱导的生长抑制，但 0.5 mM NAC 增强了 GA 诱导的 CL1-0/细胞死亡。然而，在没有 GA 的情况下，超过 2 mM 的 NAC 抑制 CL1-0/细胞生长的效果比 CL1-0/细胞更显著。在没有 GA 的情况下，NAC 具有抗氧化作用。在 GA 诱导的 ROS 应激下，NAC 可能具有促氧化作用。每种细胞类型都有一个独特的生存 ROS 水平范围。细胞内的 ROS 水平决定了细胞对抗氧化剂或促氧化剂的敏感性。使用具有不同抗氧化能力的细胞表明，细胞内的 ROS 水平影响 NAC 的功能，并为 NAC 的辅助临床应用提供了有价值的信息。
