基于下一代测序的外泌体 miRNA 与冠状动脉疾病的相关性研究。
Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing.
机构信息
Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan.
Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, CA 92093, USA.
出版信息
Cells. 2021 Dec 29;11(1):98. doi: 10.3390/cells11010098.
Abstract
BACKGROUND
Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD).
METHODS
Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD.
RESULTS
Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p.
CONCLUSION
We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.
摘要
背景
在细胞间通过外泌体传递的各种生物信息分子中，microRNAs（miRNAs）是一种非常有潜力的冠心病（CAD）潜在生物标志物候选物，因为它们即使在反复冻融后仍能保持高度稳定。
方法
从 214 名被诊断为三血管 CAD 的患者和 140 名无 CAD 的患者的冠状动脉采集血液样本。沉淀提取后，发现外泌体的数量随着年龄的增加和三血管 CAD 的增加而减少。接下来进行了下一代测序，以进一步探索外泌体 miRNAs 与 CAD 之间的可能关系。
结果
有 8 种外泌体 miRNAs 的表达与 CAD 相关发生改变。CAD 上调的 miRNAs 为 miRNA-382-3p、miRNA-432-5p、miRNA-200a-3p 和 miRNA-3613-3p。下调的 miRNAs 为 miRNA-125a-5p、miRNA-185-5p、miRNA-151a-3p 和 miRNA-328-3p。
结论
我们成功地证明了特定的外泌体 miRNAs 可能成为未来 CAD 的生物标志物。
相似文献
1
Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing.基于下一代测序的外泌体 miRNA 与冠状动脉疾病的相关性研究。
Cells. 2021 Dec 29;11(1):98. doi: 10.3390/cells11010098.
2
Identification of non-invasive biomarkers for chronic atrophic gastritis from serum exosomal microRNAs.从血清外泌体 microRNAs 中鉴定慢性萎缩性胃炎的非侵入性生物标志物。
BMC Cancer. 2019 Feb 8;19(1):129. doi: 10.1186/s12885-019-5328-7.
3
Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing.采用下一代测序技术评估肿瘤来源外泌体 miRNA 作为早期非小细胞肺癌潜在诊断生物标志物。
Clin Cancer Res. 2017 Sep 1;23(17):5311-5319. doi: 10.1158/1078-0432.CCR-17-0577. Epub 2017 Jun 12.
4
Circulating Exosomal miRNAs as Novel Biomarkers for Stable Coronary Artery Disease.循环外泌体 miRNA 作为稳定型冠状动脉疾病的新型生物标志物。
Biomed Res Int. 2020 Dec 11;2020:3593962. doi: 10.1155/2020/3593962. eCollection 2020.
5
Serum exosomal microRNA let-7i-3p as candidate diagnostic biomarker for Kawasaki disease patients with coronary artery aneurysm.血清外泌体 microRNA let-7i-3p 作为川崎病合并冠状动脉瘤患者的候选诊断生物标志物。
IUBMB Life. 2019 Jul;71(7):891-900. doi: 10.1002/iub.2015. Epub 2019 Feb 6.
6
Plasma Exosomal miRNAs in Persons with and without Alzheimer Disease: Altered Expression and Prospects for Biomarkers.患有和未患阿尔茨海默病的人群血浆外泌体中的微小RNA：表达变化及生物标志物前景
PLoS One. 2015 Oct 1;10(10):e0139233. doi: 10.1371/journal.pone.0139233. eCollection 2015.
7
Investigation of miR-26a-5p and miR-19a-3p expression levels in angiographically confirmed coronary artery disease.探讨经血管造影证实的冠心病患者中 miR-26a-5p 和 miR-19a-3p 的表达水平。
Acta Cardiol. 2023 Oct;78(8):945-956. doi: 10.1080/00015385.2023.2227484. Epub 2023 Jun 28.
8
Exosome-Derived MiRNAs as Biomarkers of the Development and Progression of Intracranial Aneurysms.外泌体衍生 miRNA 作为颅内动脉瘤发生发展的生物标志物。
J Atheroscler Thromb. 2020 Jun 1;27(6):545-610. doi: 10.5551/jat.51102. Epub 2019 Oct 10.
9
Exosomal miRNAs as circulating biomarkers for prediction of development of haematogenous metastasis after surgery for stage II/III gastric cancer.外泌体 miRNA 作为循环生物标志物，用于预测 II/III 期胃癌手术后血行转移的发生。
J Cell Mol Med. 2020 Jun;24(11):6220-6232. doi: 10.1111/jcmm.15253. Epub 2020 May 8.
10
Exosomal miRNAs as potential biomarkers for acute myocardial infarction.外泌体 miRNA 作为急性心肌梗死的潜在生物标志物。
IUBMB Life. 2020 Mar;72(3):384-400. doi: 10.1002/iub.2189. Epub 2019 Nov 1.
引用本文的文献
1
SlATG8f coordinates ethylene signaling and chloroplast turnover to drive tomato fruit ripening.SlATG8f协调乙烯信号传导和叶绿体周转以驱动番茄果实成熟。
Plant Cell Rep. 2025 Aug 22;44(9):200. doi: 10.1007/s00299-025-03578-8.
2
Exosomal non-coding RNAs: key molecules in the diagnosis and treatment of coronary artery disease.外泌体非编码RNA：冠状动脉疾病诊断和治疗中的关键分子。
PeerJ. 2025 Jun 10;13:e19352. doi: 10.7717/peerj.19352. eCollection 2025.
3
miRNA-200a suppresses GNAI1 and PLCB4 to modulate skin pigmentation in cashmere goats.微小RNA-200a通过抑制GNAI1和PLCB4来调节绒山羊的皮肤色素沉着。
Sci Rep. 2025 May 20;15(1):17456. doi: 10.1038/s41598-025-01956-6.
4
Mesenchymal stem cell exosomes therapy for the treatment of traumatic brain injury: mechanism, progress, challenges and prospects.间充质干细胞外泌体疗法治疗创伤性脑损伤：机制、进展、挑战与前景
J Transl Med. 2025 Apr 11;23(1):427. doi: 10.1186/s12967-025-06445-y.
5
Exosome-mediated effects of BRCA1 on cardiovascular artery disease.BRCA1通过外泌体介导对心血管疾病的影响。
Cell Biol Toxicol. 2025 Mar 13;41(1):59. doi: 10.1007/s10565-025-09996-4.
6
MicroRNA-197-3p Transfection: Variations in Cardiomyocyte Gene Expression with Anaesthetics Drugs in a Model of Hypoxia/Reperfusion.微小RNA-197-3p转染：缺氧/复灌注模型中麻醉药物对心肌细胞基因表达的影响
Pharmaceuticals (Basel). 2025 Jan 23;18(2):146. doi: 10.3390/ph18020146.
7
Identification of plasma miR-4505, miR-4743-5p and miR-4750-3p as novel diagnostic biomarkers for coronary artery disease in patients with type 2 diabetes mellitus: a case-control study.鉴定血浆 miR-4505、miR-4743-5p 和 miR-4750-3p 作为 2 型糖尿病患者冠心病的新型诊断生物标志物：病例对照研究。
Cardiovasc Diabetol. 2024 Jul 29;23(1):278. doi: 10.1186/s12933-024-02374-0.
8
Distinct Lipidomic Profiles between People Living with HIV Treated with E/C/F/TAF or B/F/TAF: An Open-Label Prospective Cohort Study.接受E/C/F/TAF或B/F/TAF治疗的HIV感染者之间不同的脂质组学特征：一项开放标签前瞻性队列研究。
Infect Dis Ther. 2024 Apr;13(4):727-744. doi: 10.1007/s40121-024-00943-0. Epub 2024 Mar 15.
9
Targeting myeloperoxidase limits myeloid cell immunosuppression enhancing immune checkpoint therapy for pancreatic cancer.靶向髓过氧化物酶可限制髓样细胞免疫抑制，增强胰腺癌的免疫检查点治疗。
Cancer Immunol Immunother. 2024 Feb 17;73(3):57. doi: 10.1007/s00262-024-03647-z.
10
Advances in the study of exosomes in cardiovascular diseases.心血管疾病中外泌体的研究进展
J Adv Res. 2024 Dec;66:133-153. doi: 10.1016/j.jare.2023.12.014. Epub 2023 Dec 18.
本文引用的文献
1
Association of Pericardiac Adipose Tissue With Coronary Artery Disease.心包脂肪组织与冠状动脉疾病的关系。
Front Endocrinol (Lausanne). 2021 Sep 6;12:724859. doi: 10.3389/fendo.2021.724859. eCollection 2021.
2
Association of Circulating, Inflammatory-Response Exosomal mRNAs With Acute Myocardial Infarction.循环炎症反应外泌体mRNA与急性心肌梗死的关联
Front Cardiovasc Med. 2021 Aug 19;8:712061. doi: 10.3389/fcvm.2021.712061. eCollection 2021.
3
Differential miRNAs in acute spontaneous coronary artery dissection: Pathophysiological insights from a potential biomarker.急性自发性冠状动脉夹层中的差异 microRNAs：潜在生物标志物的病理生理学见解。
EBioMedicine. 2021 Apr;66:103338. doi: 10.1016/j.ebiom.2021.103338. Epub 2021 Apr 15.
4
Exosome-Derived Mediators as Potential Biomarkers for Cardiovascular Diseases: A Network Approach.外泌体衍生介质作为心血管疾病的潜在生物标志物：一种网络方法
Proteomes. 2021 Feb 1;9(1):8. doi: 10.3390/proteomes9010008.
5
Circulating Exosomal miRNAs as Novel Biomarkers for Stable Coronary Artery Disease.循环外泌体 miRNA 作为稳定型冠状动脉疾病的新型生物标志物。
Biomed Res Int. 2020 Dec 11;2020:3593962. doi: 10.1155/2020/3593962. eCollection 2020.
6
Clinical Management of Stable Coronary Artery Disease in Patients With Type 2 Diabetes Mellitus: A Scientific Statement From the American Heart Association.《2 型糖尿病患者稳定型冠状动脉疾病的临床管理：美国心脏协会的科学声明》。
Circulation. 2020 May 12;141(19):e779-e806. doi: 10.1161/CIR.0000000000000766. Epub 2020 Apr 13.
7
Influence of lipoprotein apheresis on circulating plasma levels of miRNAs in patients with high Lp(a).脂蛋白分离术对高Lp(a)患者循环血浆中微小RNA水平的影响。
Atheroscler Suppl. 2019 Dec;40:12-16. doi: 10.1016/j.atherosclerosissup.2019.08.036.
8
PBMCs-Derived microRNA Signature as a Prethrombotic Status Discriminator in Stable Coronary Artery Disease.骨髓单个核细胞衍生 microRNA 特征可作为稳定型冠状动脉疾病的血栓前状态鉴别指标。
Thromb Haemost. 2020 Jan;120(1):121-131. doi: 10.1055/s-0039-1700518. Epub 2019 Nov 12.
9
Exosomal miRNAs as potential biomarkers for acute myocardial infarction.外泌体 miRNA 作为急性心肌梗死的潜在生物标志物。
IUBMB Life. 2020 Mar;72(3):384-400. doi: 10.1002/iub.2189. Epub 2019 Nov 1.
10
Vascular Calcification and not Arrhythmia in Idiopathic Atrial Fibrillation Associates with Sex Differences in Diabetic Microvascular Injury miRNA Profiles.特发性心房颤动中的血管钙化而非心律失常与糖尿病微血管损伤miRNA谱中的性别差异相关。
Microrna. 2019;8(2):127-134. doi: 10.2174/2211536608666181122125208.
Zotero 插件