Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan.
Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, CA 92093, USA.
Cells. 2021 Dec 29;11(1):98. doi: 10.3390/cells11010098.
Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD).
Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD.
Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p.
We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.
在细胞间通过外泌体传递的各种生物信息分子中，microRNAs（miRNAs）是一种非常有潜力的冠心病（CAD）潜在生物标志物候选物，因为它们即使在反复冻融后仍能保持高度稳定。
从 214 名被诊断为三血管 CAD 的患者和 140 名无 CAD 的患者的冠状动脉采集血液样本。沉淀提取后，发现外泌体的数量随着年龄的增加和三血管 CAD 的增加而减少。接下来进行了下一代测序，以进一步探索外泌体 miRNAs 与 CAD 之间的可能关系。
有 8 种外泌体 miRNAs 的表达与 CAD 相关发生改变。CAD 上调的 miRNAs 为 miRNA-382-3p、miRNA-432-5p、miRNA-200a-3p 和 miRNA-3613-3p。下调的 miRNAs 为 miRNA-125a-5p、miRNA-185-5p、miRNA-151a-3p 和 miRNA-328-3p。
我们成功地证明了特定的外泌体 miRNAs 可能成为未来 CAD 的生物标志物。
