Hata-Mizuno Miki, Uchino Yuichi, Uchino Miki, Shimmura Shigeto, Ogawa Yoko, Tsubota Kazuo, Negishi Kazuno
Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, Japan.
J Clin Med. 2021 Dec 23;11(1):66. doi: 10.3390/jcm11010066.
This study aimed to investigate the relationship between the severity of dry eye disease (DED) and galectin-3 concentration (gal-3) and its cleavage (gal-3C) in tear fluid. Twenty-eight DED patients and 14 controls were recruited at Keio University Hospital. The lissamine green conjunctival staining (LG) score, fluorescein corneal staining (FL) score, tear film break-up time (TBUT), Schirmer's test, and ocular symptoms questionnaire score (dry eye questionnaire score, DEQS) were evaluated. Furthermore, the correlation between these parameters and the concentrations of gal-3 in tears (ng/µg) and the detection rate of gal-3C (%) were analyzed. Gal-3 concentration in tears was positively correlated with the LG score (R = 0.60, < 0.01), FL score (R = 0.49, < 0.01), and DEQS (R = 0.45, < 0.01), and negatively correlated with the TBUT score (R = -0.40, < 0.01) and Schirmer's I value (R = -0.36, < 0.01). The detection rate of gal-3C in tears was significantly associated with the severity of DED, especially with the LG ( < 0.01) and FL ( < 0.01) scores. Therefore, the concentration of gal-3 and the detection rate of gal-3C in tears had a significant relationship with the severity of ocular surface barrier disruption.
本研究旨在探讨干眼疾病（DED）的严重程度与泪液中半乳糖凝集素-3浓度（gal-3）及其裂解产物（gal-3C）之间的关系。在庆应义塾大学医院招募了28例DED患者和14名对照者。评估了丽丝胺绿结膜染色（LG）评分、荧光素角膜染色（FL）评分、泪膜破裂时间（TBUT）、Schirmer试验以及眼部症状问卷评分（干眼问卷评分，DEQS）。此外，分析了这些参数与泪液中gal-3浓度（ng/µg）以及gal-3C检测率（%）之间的相关性。泪液中gal-3浓度与LG评分（R = 0.60，P < 0.01）、FL评分（R = 0.49，P < 0.01）和DEQS（R = 0.45，P < 0.01）呈正相关，与TBUT评分（R = -0.40，P < 0.01）和Schirmer I值（R = -0.36，P < 0.01）呈负相关。泪液中gal-3C的检测率与DED的严重程度显著相关，尤其是与LG（P < 0.01）和FL（P < 0.01）评分相关。因此，泪液中gal-3的浓度和gal-3C的检测率与眼表屏障破坏的严重程度有显著关系。
