Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
Biol Reprod. 2019 Nov 21;101(5):986-1000. doi: 10.1093/biolre/ioz137.
SALL1 and SALL3 are transcription factors that play an essential role in regulating developmental processes and organogenesis in many species. However, the functional role of SALL1 and SALL3 in chicken prehierarchical follicle development is unknown. This study aimed to explore the potential role and mechanism of csal1 and csal3 in granulosa cell proliferation, differentiation, and follicle selection within the prehierarchical follicles of hen ovary. Our data demonstrated that the csal1 and csal3 transcriptions were highly expressed in granulosa cells of prehierarchical follicles, and their proteins were mainly localized in the cytoplasm of granulosa cells and oocytes as well as in the ovarian stroma and epithelium. It initially revealed that both csal1 and csal3 may be involved in chicken prehierarchical follicle development via a translocation mechanism. Furthermore, our results showed an abundance of CCND1, Bcat, StAR, CYP11A1, and FSHR mRNA in granulosa cells, and the proliferation levels of granulosa cells from the prehierarchical follicles were significantly increased by siRNA-mediated knockdown of csal1 or/and csal3. Conversely, the overexpression of csal1 or/and csal3 in the granulosa cells led to a remarkably decreased of them. Moreover, csal1 and csal3 together exert a much stronger effect on the regulation than any of csal1 or csal3. These results indicated that csal1 and csal3 play synergistic inhibitory roles on granulosa cell proliferation, differentiation, and steroidogenesis during prehierarchical follicle development in vitro. The current data provide a basis of molecular mechanisms of csal1 and csal3 in controlling the prehierarchical follicle development and growth of hen ovary in vivo.
SALL1 和 SALL3 是转录因子，在许多物种的发育过程和器官发生中发挥着重要作用。然而，SALL1 和 SALL3 在鸡前层级卵泡发育中的功能作用尚不清楚。本研究旨在探讨 csal1 和 csal3 在母鸡卵巢前层级卵泡中颗粒细胞增殖、分化和卵泡选择中的潜在作用和机制。我们的数据表明，csal1 和 csal3 转录物在前层级卵泡的颗粒细胞中高度表达，其蛋白主要定位于颗粒细胞和卵母细胞的细胞质以及卵巢基质和上皮中。初步揭示 csal1 和 csal3 可能通过易位机制参与鸡前层级卵泡发育。此外，我们的结果显示，在颗粒细胞中存在丰富的 CCND1、Bcat、StAR、CYP11A1 和 FSHR mRNA，并且通过 siRNA 介导的 csal1 或/和 csal3 敲低显著增加了前层级卵泡颗粒细胞的增殖水平。相反，csal1 或/和 csal3 在颗粒细胞中的过表达导致其水平显著降低。此外，csal1 和 csal3 一起对调控的作用比 csal1 或 csal3 中的任何一个都要强。这些结果表明，csal1 和 csal3 在体外前层级卵泡发育过程中对颗粒细胞增殖、分化和类固醇生成具有协同抑制作用。这些数据为 csal1 和 csal3 控制母鸡卵巢前层级卵泡发育和生长的分子机制提供了基础。
