Muğla Sıtkı Koçman University, Faculty of Health Sciences, Muğla, 48000, Turkey.
Muğla Sıtkı Koçman University, Research Laboratories Center, Muğla, 48000, Turkey.
Immunotherapy. 2022 Mar;14(4):225-247. doi: 10.2217/imt-2021-0174. Epub 2022 Jan 11.
In this article, the authors investigate the modulatory effects of dental mesenchymal stem cells (MSCs) on lymphocyte responses in primary Sjögren's syndrome (pSS), which is an autoimmune disease resulting from keratoconjunctivitis sicca and xerostomia. Mononuclear cells isolated from pSS patients cultured with or without dental MSCs and analyzed for lymphocyte responses via flow cytometry. Dental-follicle (DF)- and dental-pulp (DP)-MSCs downregulated CD4 T lymphocyte proliferation by increasing Fas-ligand expression on T lymphocytes and FoxP3 expressing Tregs, and decreasing intracellular IFN-γ and IL-17 secretion in pSS patients. DF-MSCs decreased the plasma B cell ratio in the favor of naive B cell population in pSS patients' mononuclear cells. DF- and DP-MSCs can be the new cellular therapeutic candidates for the regulation of immune responses in pSS.
本文作者研究了牙髓间充质干细胞（MSCs）对原发性干燥综合征（pSS）患者淋巴细胞反应的调节作用。pSS 是一种自身免疫性疾病，表现为眼干和口干。作者从 pSS 患者中分离出单核细胞，在有无牙髓 MSCs 的情况下进行培养，并通过流式细胞术分析淋巴细胞反应。结果表明，牙囊（DF）-和牙髓（DP）-MSCs 通过增加 T 细胞上 Fas 配体的表达和 FoxP3 表达的调节性 T 细胞（Tregs），以及减少 pSS 患者 T 细胞内 IFN-γ 和 IL-17 的分泌，抑制 CD4 T 淋巴细胞的增殖。DF-MSCs 降低了 pSS 患者单核细胞中浆细胞的比例，有利于幼稚 B 细胞群的增加。DF 和 DP-MSCs 可能成为调节 pSS 免疫反应的新型细胞治疗候选物。
