Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
University of Florida Racing Laboratory, Gainesville, FL, USA.
J Vet Diagn Invest. 2022 Mar;34(2):226-230. doi: 10.1177/10406387211070539. Epub 2022 Jan 10.
Pentobarbital is used commonly to euthanize animals. Occasionally during a death investigation, it is necessary to determine whether a cat or dog was euthanized via pentobarbital overdose. Screening for the detection of barbiturates including pentobarbital can be performed using commercial immunochromatographic tests. We used a commercial immunochromatographic test for barbiturates in humans to screen for barbiturates in urine collected postmortem from 20 dogs and 20 cats to determine whether they had been euthanized with pentobarbital. Additionally, we analyzed the urine for pentobarbital using liquid chromatography-mass spectrometry as a confirmatory test. Screening and confirmation testing revealed 100% agreement between the tests and with the euthanasia status of each animal. Our results support the use of the immunochromatographic test for the screening of urine collected postmortem to assess for the presence of barbiturates, specifically pentobarbital, used for euthanasia.
戊巴比妥常用于动物安乐死。偶尔在死亡调查中，有必要确定猫或狗是否因戊巴比妥过量而安乐死。可以使用商业免疫层析试验来筛选检测包括戊巴比妥在内的巴比妥类药物。我们使用一种商业的人类巴比妥类免疫层析检测试剂盒，对 20 只狗和 20 只猫死后采集的尿液进行筛选，以确定它们是否被戊巴比妥安乐死。此外，我们还使用液相色谱-质谱法对尿液中的戊巴比妥进行了分析，作为确认试验。筛查和确认试验结果表明，检测方法与每只动物的安乐死状态之间具有 100%的一致性。我们的结果支持使用免疫层析试验对死后采集的尿液进行筛选，以评估用于安乐死的巴比妥类药物（特别是戊巴比妥）的存在。
