Central Laboratory, ZhongShan Hospital XiaMen University, Xiamen, Fujian, China.
Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Zhongshan Hospital of Xiamen University, Xiamen, Fujian, China.
Bioengineered. 2022 Jan;13(1):1767-1778. doi: 10.1080/21655979.2021.1995578.
Osteosarcoma (OS) is the most common primary malignant tumor of bone mainly occurring in children and young people, which has a high rate of recurrence and metastasis. Long non-coding RNAs (lncRNAs) have capabilities in regulating target gene expression in various tumors served as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs). In addition, Ezrin (EZR) is a member of ERM (ezrin/Radixin/moesin) protein family that contributes to the progression of multiple tumors. Previous studies have correlated lncRNA taurine upregulated 1 (TUG1) or Ezrin with OS. However, the correlation between lncRNA TUG1 and Ezrin in OS remains unclear. The expressions of lncRNA TUG1 and Ezrin were upregulated in OS tissues and cells determined by quantitative reverse transcription-PCR (qRT-PCR) and Western blot (WB), respectively. In addition, both lncRNA TUG1 and Ezrin promoted OS cell proliferation identified by Cell Counting Kit-8 (CCK-8) assay and clone formation assay, and enhanced OS cell invasion detected using Transwell assay for cell invasion. Moreover, lncRNA TUG1 upregulated Ezrin expression through sponging miR-377-3p determined by dual-luciferase reporter gene assay and WB. In conclusion, our work revealed that lncRNA TUG1 promoted OS cell proliferation and invasion through upregulating Ezrin expression as a ceRNA of miR-377-3p, which might provide novel therapeutic targets for OS therapy.
骨肉瘤（OS）是最常见的原发性骨恶性肿瘤，主要发生在儿童和年轻人中，具有较高的复发和转移率。长链非编码 RNA（lncRNA）在各种肿瘤中作为竞争性内源性 RNA（ceRNA）调节靶基因表达，发挥作用。此外，Ezrin（EZR）是 ERM（ezrin/Radixin/moesin）蛋白家族的一员，有助于多种肿瘤的进展。先前的研究已经将 lncRNA 牛磺酸上调 1（TUG1）或 Ezrin 与 OS 相关联。然而，lncRNA TUG1 和 Ezrin 在 OS 中的相关性尚不清楚。通过定量逆转录-PCR（qRT-PCR）和 Western blot（WB）分别确定 lncRNA TUG1 和 Ezrin 在 OS 组织和细胞中表达上调。此外，通过细胞计数试剂盒-8（CCK-8）测定和克隆形成测定确定 lncRNA TUG1 和 Ezrin 均促进 OS 细胞增殖，通过 Transwell 测定评估细胞侵袭检测到 OS 细胞侵袭增强。此外，通过双荧光素酶报告基因测定和 WB 确定 lncRNA TUG1 通过海绵 miR-377-3p 上调 Ezrin 表达。总之，我们的工作表明，lncRNA TUG1 通过作为 miR-377-3p 的 ceRNA 上调 Ezrin 表达促进 OS 细胞增殖和侵袭，这可能为 OS 治疗提供新的治疗靶点。
