弓形虫裂解周期中宿主免疫和代谢反应相关长非编码 RNA 和信使 RNA 的时间转录组变化。
Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle.
机构信息
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.
出版信息
Parasit Vectors. 2022 Jan 10;15(1):22. doi: 10.1186/s13071-021-05140-3.
BACKGROUND
Long non-coding RNAs (lncRNAs) are important regulators of various biological and pathological processes, in particular the inflammatory response by modulating the transcriptional control of inflammatory genes. However, the role of lncRNAs in regulating the immune and inflammatory responses during infection with the protozoan parasite Toxoplasma gondii remains largely unknown.
METHODS
We performed a longitudinal RNA sequencing analysis of human foreskin fibroblast (HFF) cells infected by T. gondii to identify differentially expressed long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs), and dysregulated pathways over the course of T. gondii lytic cycle. The transcriptome data were validated by qRT-PCR.
RESULTS
RNA sequencing revealed significant transcriptional changes in the infected HFFs. A total of 697, 1234, 1499, 873, 1466, 561, 676 and 716 differentially expressed lncRNAs (DElncRNAs), and 636, 1266, 1843, 2303, 3022, 1757, 3088 and 2531 differentially expressed mRNAs (DEmRNAs) were identified at 1.5, 3, 6, 9, 12, 24, 36 and 48 h post-infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DElncRNAs and DEmRNAs revealed that T. gondii infection altered the expression of genes involved in the regulation of host immune response (e.g., cytokine-cytokine receptor interaction), receptor signaling (e.g., NOD-like receptor signaling pathway), disease (e.g., Alzheimer's disease), and metabolism (e.g., fatty acid degradation).
CONCLUSIONS
These results provide novel information for further research on the role of lncRNAs in immune regulation of T. gondii infection.
背景
长非编码 RNA（lncRNA）是各种生物和病理过程的重要调节因子，特别是通过调节炎症基因的转录控制来调节炎症反应。然而，lncRNA 在调节感染原虫弓形虫时的免疫和炎症反应中的作用在很大程度上仍然未知。
方法
我们对感染弓形虫的人包皮成纤维细胞（HFF）进行了纵向 RNA 测序分析，以鉴定在弓形虫裂解周期过程中差异表达的长非编码 RNA（lncRNA）和信使 RNA（mRNA）以及失调的途径。通过 qRT-PCR 验证了转录组数据。
结果
RNA 测序显示感染 HFF 中的转录发生了显著变化。总共鉴定出 697、1234、1499、873、1466、561、676 和 716 个差异表达的 lncRNA（DElncRNA），以及 636、1266、1843、2303、3022、1757、3088 和 2531 个差异表达的 mRNA（DEmRNA），分别在感染后 1.5、3、6、9、12、24、36 和 48 小时。DElncRNA 和 DEmRNA 的基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析显示，弓形虫感染改变了宿主免疫反应调节（例如细胞因子-细胞因子受体相互作用）、受体信号（例如 NOD 样受体信号通路）、疾病（例如阿尔茨海默病）和代谢（例如脂肪酸降解）中涉及的基因的表达。
结论
这些结果为进一步研究 lncRNA 在弓形虫感染免疫调节中的作用提供了新的信息。
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