University of Bordeaux, INRAE, BIOGECO, 33615, Pessac, France.
BMC Res Notes. 2022 Jan 10;15(1):15. doi: 10.1186/s13104-021-05903-2.
Angelica heterocarpa (Apiaceae) is a wild endemic French species with special conservation interest in the European Union. It belongs to Angelica complex genus which is widespread throughout the north temperate zone, and is sympatric with other congeneric species. The objective of this work is to develop and characterize microsatellite markers as a new tool for understanding the ecology and evolution of Angelica species complex.
We identified simple sequence repeat (SSR) regions in a microsatellite-enriched library from A. heterocarpa and A. sylvestris. All 16 selected SSR regions were found to amplify in these species and were highly polymorphic. Marker transferability was validated in A. razulii and A. archangelica. These markers will help us to better understand the evolutionary dynamic between rare endemics and widespread sister species, and be useful for conservation of the endemic species. Moreover, they can provide new tools for studying the numerous traditional medicinal herbs of the Angelica genus.
独活（伞形科）是一种法国特有野生植物，在欧盟具有特殊的保护意义。它属于广泛分布于北温带的独活属植物复合体，与其他同属种共存。本研究旨在开发和鉴定微卫星标记，作为研究独活属植物复合体生态和进化的新工具。
我们从独活和野独活的微卫星富集文库中鉴定出简单序列重复（SSR）区域。在这两个物种中，所有 16 个选定的 SSR 区域都能扩增，并具有高度多态性。标记的可转移性在 Razulii 独活和 angelica 当归中得到了验证。这些标记将有助于我们更好地理解稀有特有种与广泛分布的姐妹种之间的进化动态，对特有种的保护也具有重要意义。此外，它们还可以为研究当归属的众多传统药用植物提供新的工具。
