濒危特有物种新型简单重复序列（SSR）标记的开发与特性分析
Development and characterization of novel SSR markers in the endangered endemic species .
作者信息
Malkócs Tamás, Laczkó Levente, Bereczki Judit, Meglécz Emese, Szövényi Péter, Sramkó Gábor
机构信息
Department of Botany University of Debrecen Debrecen Hungary.
Department of Evolutionary Zoology and Human Biology University of Debrecen Debrecen Hungary.
出版信息
Appl Plant Sci. 2020 Feb 11;8(2):e11321. doi: 10.1002/aps3.11321. eCollection 2020 Feb.
PREMISE
(Apiaceae) is a polycarpic, perennial herb with a very limited range and small populations. It is listed as "endangered" on the IUCN Red List of Threatened Species. Microsatellite markers can contribute to conservation efforts by allowing the study of the genetic structure of its shrinking populations.
METHODS AND RESULTS
We used a microsatellite-enriched library combined with an Illumina sequencing approach to develop simple sequence repeat markers in our target species. Out of 44 tested primer pairs, 22 provided specific products, and 13 showed heterologous amplification in the target species. Cross-species amplification was achieved at 20 and 19 loci in two congeneric species, and , respectively.
CONCLUSIONS
The primers described here are the first tools that enable the population genetic characterization of . Our results suggest a wider applicability in the genus .
前提
（伞形科）是一种多次结果的多年生草本植物，分布范围非常有限，种群数量少。它在世界自然保护联盟濒危物种红色名录中被列为“濒危”物种。微卫星标记可以通过研究其不断缩小的种群的遗传结构，为保护工作做出贡献。
方法与结果
我们使用了一个富集微卫星的文库，并结合Illumina测序方法，在我们的目标物种中开发简单序列重复标记。在44对测试引物中，22对产生了特异性产物，13对在目标物种中显示出异源扩增。在两个同属物种中，分别在20个和19个位点实现了跨物种扩增。
结论
这里描述的引物是第一批能够对进行种群遗传特征分析的工具。我们的结果表明，它们在该属中具有更广泛的适用性。
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