Su B, Cao L, Zheng J, Zhang L, Zhang Z, Qiu F
Department of Clinical Laboratory, Zhongshan Torch Development Zone Hospital, Zhongshan 528437, China.
Department of Laboratory Medicine, Seventh Affiliated Hospital of Southern Medical University, Foshan 528244, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Dec 20;41(12):1809-1815. doi: 10.12122/j.issn.1673-4254.2021.12.08.
To characterize the bacterial strain LGV03 isolated from the reproductive tract of healthy women and evaluate its safety.
The bacterial strains were isolated and purified using MRS agar by dilution plating and streak plate method. Gram staining was used to observe the morphology of the isolated strains and identify Gram-negative and -positive strains. The physiological and biochemical properties of the bacteria was assessed using VITEK ANC card. Strain LGV03 was identified by molecular biology and phylogenetic analysis and tested for hemolysis on blood agar plates. E-test method was performed to examine the sensitivity of strain LGV03. The concentration of lactic acid in the culture supernatant of LGV03 was measured using a biochemical analyzer. The safety of LGV03 was evaluated in mice using acute oral toxicity test.
The isolated bacterial strain LGV03 showed good growth under anaerobic conditions at 37 ℃ on MRS medium and formed round, milky white, moist, and opaque colonies with neat margins. The strain LGV03 was positive for Gram staining, rod-shaped with a size range of (0.4-0.5) × (0.9-6.3) μm, and was identified as . LGV03 was sensitive to PG, AM, MP, VA, EM and LZ. In MRS medium LGV03 could produce lactic acid, which reached its saturation concentration after 18 h with a final concentration of 1.72 mg/mL. The mice with acute oral exposure to LGV03 showed no significant changes of body weight or organ weight as compared with normal saline-treated mice.
The bacterial strain LGV03 isolated from the female reproductive tract was identified as with potential probiotic effects and good safety profile.
对从健康女性生殖道分离出的细菌菌株LGV03进行特性分析并评估其安全性。
采用稀释平板法和划线平板法，使用MRS琼脂对细菌菌株进行分离和纯化。通过革兰氏染色观察分离菌株的形态，鉴定革兰氏阴性和阳性菌株。使用VITEK ANC卡评估细菌的生理生化特性。通过分子生物学和系统发育分析鉴定菌株LGV03，并在血琼脂平板上检测其溶血情况。采用E-test法检测菌株LGV03的敏感性。使用生化分析仪测量LGV03培养上清液中乳酸的浓度。通过急性经口毒性试验在小鼠中评估LGV03的安全性。
分离出的细菌菌株LGV03在MRS培养基上于37℃厌氧条件下生长良好，形成边缘整齐的圆形、乳白色、湿润且不透明的菌落。菌株LGV03革兰氏染色呈阳性，杆状，大小范围为(0.4 - 0.5)×(0.9 - 6.3)μm，被鉴定为 。LGV03对PG、AM、MP、VA、EM和LZ敏感。在MRS培养基中，LGV03可产生乳酸，18小时后达到饱和浓度，终浓度为1.72mg/mL。急性经口暴露于LGV03的小鼠与生理盐水处理的小鼠相比，体重和器官重量无显著变化。
从女性生殖道分离出的细菌菌株LGV03被鉴定为 ，具有潜在的益生菌作用且安全性良好。
