Dixon J F, Hokin L E
Department of Pharmacology, University of Wisconsin Medical School, Madison 53706.
Biochem Biophys Res Commun. 1987 Dec 31;149(3):1208-13. doi: 10.1016/0006-291x(87)90536-5.
Hawkins et al. [Hawkins, P.T., Berrie, C.P., Morris, A.J., and Downes, C.P. (1987) Biochem J. 243, 211-218] were unable to find any formation of inositol 1,2-cyclic 4,5-trisphosphate on muscarinic stimulation of rat parotid slices, contrary to what has been found in mouse pancreas and in platelets. We have repeated the studies of Hawkins et al. using [3H]inositol-prelabelled rat parotid minilobules and our improved HPLC method for clearly separating the three inositol trisphosphates. Substantial amounts of inositol 1,2-cyclic 4,5-trisphosphate formed on muscarinic stimulation of rat parotid minilobules, amounting to 5% of inositol 1,4,5-trisphosphate at 10 sec and one third of inositol 1,4,5-trisphosphate at 5 min.
霍金斯等人[霍金斯，P.T.，贝里，C.P.，莫里斯，A.J.，唐斯，C.P.（1987年）《生物化学杂志》243卷，211 - 218页]在对大鼠腮腺切片进行毒蕈碱刺激时，未能发现有任何肌醇1,2 - 环4,5 - 三磷酸的形成，这与在小鼠胰腺和血小板中发现的情况相反。我们使用[³H]肌醇预标记的大鼠腮腺小叶以及我们改进的用于清晰分离三种肌醇三磷酸的高效液相色谱方法，重复了霍金斯等人的研究。在对大鼠腮腺小叶进行毒蕈碱刺激时，形成了大量的肌醇1,2 - 环4,5 - 三磷酸，在10秒时相当于肌醇1,4,5 - 三磷酸的5%，在5分钟时相当于肌醇1,4,5 - 三磷酸的三分之一。
