在激动剂刺激的腮腺中，肌醇1,3,4,5-四磷酸而非磷脂酰肌醇3,4-二磷酸可能是肌醇1,3,4-三磷酸的前体。
Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.
作者信息
Downes C P, Hawkins P T, Irvine R F
出版信息
Biochem J. 1986 Sep 1;238(2):501-6. doi: 10.1042/bj2380501.
Abstract
When [3H]inositol-prelabelled rat parotid-gland slices were stimulated with carbachol, noradrenaline or Substance P, the major inositol trisphosphate produced with prolonged exposure to agonists was, in each case, inositol 1,3,4-trisphosphate. Much lower amounts of radioactivity were present in the inositol 1,4,5-trisphosphate fraction separated by anion-exchange h.p.l.c. Analysis of the inositol trisphosphate head group of phosphatidylinositol bisphosphate in [32P]Pi-labelled parotid glands showed the presence of phosphatidylinositol 4,5-bisphosphate, but no detectable phosphatidylinositol 3,4-bisphosphate. Carbachol-stimulated [3H]inositol-labelled parotid glands contained an inositol polyphosphate with the chromatographic properties and electrophoretic mobility of an inositol tetrakisphosphate, the probable structure of which was determined to be inositol 1,3,4,5-tetrakisphosphate. Since an enzyme in erythrocyte membranes is capable of degrading this tetrakisphosphate to inositol 1,3,4-trisphosphate, it is suggested to be the precursor of inositol 1,3,4-trisphosphate in parotid glands.
摘要
当用卡巴胆碱、去甲肾上腺素或P物质刺激经[³H]肌醇预标记的大鼠腮腺切片时，在长时间暴露于激动剂的情况下产生的主要肌醇三磷酸，在每种情况下均为肌醇1,3,4-三磷酸。通过阴离子交换高效液相色谱法分离得到的肌醇1,4,5-三磷酸部分中的放射性含量要低得多。对[³²P]Pi标记的腮腺中磷脂酰肌醇二磷酸的肌醇三磷酸头部基团的分析表明存在磷脂酰肌醇4,5-二磷酸，但未检测到磷脂酰肌醇3,4-二磷酸。卡巴胆碱刺激的[³H]肌醇标记的腮腺含有一种肌醇多磷酸，其具有肌醇四磷酸的色谱特性和电泳迁移率，其可能的结构被确定为肌醇1,3,4,5-四磷酸。由于红细胞膜中的一种酶能够将这种四磷酸降解为肌醇1,3,4-三磷酸，因此它被认为是腮腺中肌醇1,3,4-三磷酸的前体。
相似文献
1
Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.在激动剂刺激的腮腺中，肌醇1,3,4,5-四磷酸而非磷脂酰肌醇3,4-二磷酸可能是肌醇1,3,4-三磷酸的前体。
Biochem J. 1986 Sep 1;238(2):501-6. doi: 10.1042/bj2380501.
2
Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.大鼠腮腺中肌醇1,3,4,5 - 四磷酸和肌醇1,3,4 - 三磷酸的快速形成可能均间接源于受体刺激下磷脂酰肌醇4,5 - 二磷酸释放出肌醇1,4,5 - 三磷酸。
Biochem J. 1986 Sep 1;238(2):507-16. doi: 10.1042/bj2380507.
3
Inositol 1,2-cyclic 4,5-trisphosphate is not a product of muscarinic receptor-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis in rat parotid glands.肌醇1,2-环4,5-三磷酸不是毒蕈碱受体刺激大鼠腮腺中磷脂酰肌醇4,5-二磷酸水解的产物。
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4
Inositol trisphosphates in carbachol-stimulated rat parotid glands.卡巴胆碱刺激的大鼠腮腺中的肌醇三磷酸
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5
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6
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7
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8
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9
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Myo-inositol phosphates obtained by alkaline hydrolysis of beef brain phosphoinositide.通过牛肉脑磷酸肌醇碱性水解得到的肌醇磷酸酯。
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Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.1,4,5-三磷酸肌醇促使胰腺腺泡细胞非线粒体胞内钙库释放钙离子。
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Inositol trisphosphates in carbachol-stimulated rat parotid glands.卡巴胆碱刺激的大鼠腮腺中的肌醇三磷酸
Biochem J. 1984 Oct 1;223(1):237-43. doi: 10.1042/bj2230237.
9
Breakdown of polyphosphoinositides and not phosphatidylinositol accounts for muscarinic agonist-stimulated inositol phospholipid metabolism in rat parotid glands.多磷酸肌醇而非磷脂酰肌醇的分解代谢是毒蕈碱激动剂刺激大鼠腮腺中肌醇磷脂代谢的原因。
Biochem J. 1983 Dec 15;216(3):633-40. doi: 10.1042/bj2160633.
10
Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.膜磷酸肌醇在激动剂依赖性水解后肌醇磷酸水平的变化。
Biochem J. 1983 May 15;212(2):473-82. doi: 10.1042/bj2120473.
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