基质血管成分对注射软骨碎块血管生成的影响。
Effect of Stromal Vascular Fractions on Angiogenesis of Injected Diced Cartilage.
机构信息
Departments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Departments of Plastic Surgery, The Second Affiliated Hospital of Hainan Medical University, Haikou.
出版信息
J Craniofac Surg. 2022;33(2):713-718. doi: 10.1097/SCS.0000000000007996.
OBJECTIVES
This study explored the effect of adipose-derived stromal vascular fractions (SVFs) on angiogenesis in injected autologous diced cartilage.
METHODS
Stromal vascular fractions were extracted by enzymatic digestion. Cartilage grafts were harvested from 1 side of the auricular cartilage of New Zealand rabbit and then diced to a size of 1.0 mm3. The grafts were divided into 2 groups. The control group was diced cartilage mixed with culture medium, and the experimental group was diced cartilage mixed with SVFs. The 2 groups of composite grafts were subcutaneously implanted on both sides of the back of each rabbit. After 4, 12 and 24 weeks, the tissue structure, number of blood vessels, and angiogenic factors in the grafts were observed.
RESULTS
The SVFs conformed to the current standard of the biological evaluation. Under an inverted microscope, the number of layers of chondrocytes in the experimental group was higher than that in the control group at 4 weeks. A small number of inflammatory cells and blood vessels were observed around the cartilage grafts. At 12 and 24 weeks, the volume of tissue was increased gradually by general observation. And a large number of chondrocytes were observed microscopically, whereas the number of inflammatory cells decreased. And meanwhile additional new blood vessels were observed. Immunohistochemical analysis of CD31 showed that the number of capillaries in the control group was significantly lower than that in the experimental group at 4, 12 and 24 weeks. Further, the expression of Hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) mRNA and protein were measured by RT-PCR and Western bloting, respectively. The results showed that the mRNA expression of VEGF and HIF-1α in the experimental group was increased. The mRNA level remained higher than that of the control group at 24 weeks (P < 0.05). And the relative expression levels of VEGF and HIF-1α protein in the experimental group were higher than those in the control group at 4, 12 and 24 weeks (P < 0.05).
CONCLUSION
Autologous diced cartilage mixed with adipose-derived SVFs can promote angiogenesis when transplanted by injection. Further research showed that SVFs could increase the expression levels of VEGF and HIF-1α in the grafts, which may be part of the mechanism that SVFs promoted the angiogenesis of diced cartilage.
目的
本研究探讨了脂肪来源的基质血管成分（SVF）对注射自体软骨碎块中血管生成的影响。
方法
通过酶消化提取基质血管成分。从新西兰兔耳软骨的一侧切取软骨移植物，切成 1.0mm3 的大小。将移植物分为两组。对照组为软骨碎块与培养基混合，实验组为软骨碎块与 SVF 混合。两组复合移植物分别皮下植入每只兔背部两侧。4、12 和 24 周后，观察移植物的组织结构、血管数量和血管生成因子。
结果
SVF 符合当前生物学评价标准。在倒置显微镜下，实验组软骨细胞层数高于对照组 4 周。软骨移植物周围观察到少量炎性细胞和血管。12 和 24 周时，大体观察到组织体积逐渐增加。显微镜下观察到大量软骨细胞，炎性细胞数量减少。同时观察到更多的新血管。CD31 的免疫组织化学分析显示，4、12 和 24 周时对照组毛细血管数量明显低于实验组。进一步通过 RT-PCR 和 Western blot 分别测量缺氧诱导因子-1（HIF-1）和血管内皮生长因子（VEGF）mRNA 和蛋白的表达。结果显示实验组 VEGF 和 HIF-1α 的 mRNA 表达增加。24 周时实验组 mRNA 水平仍高于对照组（P<0.05）。实验组 VEGF 和 HIF-1α 蛋白的相对表达水平在 4、12 和 24 周时均高于对照组（P<0.05）。
结论
自体软骨碎块与脂肪来源的 SVF 混合注射移植可促进血管生成。进一步研究表明，SVF 可增加移植物中 VEGF 和 HIF-1α 的表达水平，这可能是 SVF 促进软骨碎块血管生成的部分机制。
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