Bony Claire, Cren Mailys, Domergue Sophie, Toupet Karine, Jorgensen Christian, Noël Danièle
U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; UFR de Médecine, Montpellier University, Montpellier, France.
U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; UFR de Médecine, Montpellier University, Montpellier, France; Chirurgie Plastique Reconstructrice et Esthétique, Hôpital Gui de Chauliac, Montpellier, France.
Front Immunol. 2016 Jan 18;6:655. doi: 10.3389/fimmu.2015.00655. eCollection 2015.
Mesenchymal stem or stromal cells (MSC) are under investigation in many clinical trials for their therapeutic potential in a variety of diseases, including autoimmune and inflammatory disorders. One of the main sources of MSCs is the adipose tissue, which is mainly obtained by manual liposuction using a cannula linked to a syringe. However, in the past years, a number of devices for fat liposuction intended for clinical use have been commercialized but few papers have compared these procedures in terms of stromal vascular fraction (SVF) or adipose mesenchymal stromal cells (ASC). The objective of the present study was to compare and qualify for clinical use the ASC obtained from fat isolated with the manual or the Bodyjet(®) water-jet-assisted procedure. Although the initial number of cells obtained after collagenase digestion was higher with the manual procedure, the percentage of dead cells, the number of colony forming unit-fibroblast and the phenotype of cells were identical in the SVF at isolation (day 0) and in the ASC populations at day 14. We also showed that the osteogenic and adipogenic differentiation potentials of ASCs were identical between preparations while a slight but significant higher in vitro immunosuppressive effect was observed with ASCs isolated from fat removed with a cannula. The difference in the immunomodulatory effect between ASC populations was, however, not observed in vivo using the delayed-type hypersensitivity (DTH) model. Our data, therefore, indicate that the procedure for fat liposuction does not impact the characteristics or the therapeutic function of ASCs.
间充质干细胞（MSC）正在许多临床试验中接受研究，以探究其在包括自身免疫性和炎症性疾病在内的多种疾病中的治疗潜力。MSC的主要来源之一是脂肪组织，主要通过使用连接注射器的套管进行手动抽脂获取。然而，在过去几年中，一些用于临床的脂肪抽脂设备已商业化，但很少有论文在基质血管成分（SVF）或脂肪间充质干细胞（ASC）方面对这些方法进行比较。本研究的目的是比较并鉴定通过手动或水动力辅助抽脂法（Bodyjet®）从脂肪中获得的ASC用于临床的情况。尽管胶原酶消化后手动抽脂法获得的初始细胞数量更多，但在分离时（第0天）的SVF以及第14天的ASC群体中，死细胞百分比、成纤维细胞集落形成单位数量和细胞表型均相同。我们还表明，不同制备方法之间ASC的成骨和成脂分化潜能相同，而用套管抽脂获得的脂肪分离出的ASC在体外具有轻微但显著更高的免疫抑制作用。然而，使用迟发型超敏反应（DTH）模型在体内未观察到ASC群体之间免疫调节作用的差异。因此，我们的数据表明脂肪抽脂方法不会影响ASC的特性或治疗功能。
