Department and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
Center for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Sci Rep. 2022 Jan 10;12(1):432. doi: 10.1038/s41598-021-03155-5.
Behavioral measurements in mice are critical tools used to evaluate the effects of interventions. Whilst mice are nocturnal animals, many studies conduct behavioral tests during the day. To better understand the effects of diurnal rhythm on mouse behaviors, we compared the results from behavioral tests conducted in the active and inactive phases. C57BL/6 mice were used in this study; we focus on sensorimotor performance, anxiety, learning and memory. Overall, our results show mice exhibit slightly higher cutaneous sensitivity, better long-term contextual memory, and a greater active avoidance escape response during the active phase. We did not observe significant differences in motor coordination, anxiety, or spatial learning and memory. Furthermore, apart from the elevated-O-maze, there was no remarkable sex effect among these tests. This study provides information on the effects of different diurnal phases on types of behavior and demonstrates the importance of the circadian cycle on learning and memory. Although we did not detect differences in anxiety and spatial learning/memory, diurnal rhythm may interact with other factors to influence these behaviors.
在小鼠中进行行为测量是评估干预措施效果的重要工具。虽然小鼠是夜行性动物，但许多研究在白天进行行为测试。为了更好地理解昼夜节律对小鼠行为的影响，我们比较了在活动期和非活动期进行的行为测试的结果。本研究使用了 C57BL/6 小鼠；我们专注于感觉运动表现、焦虑、学习和记忆。总的来说，我们的结果表明，在活动期，小鼠表现出稍高的皮肤敏感性、更好的长期情景记忆和更高的主动回避逃避反应。我们没有观察到运动协调、焦虑或空间学习和记忆方面的显著差异。此外，除了高架 O 迷宫测试外，这些测试中没有明显的性别效应。这项研究提供了关于不同昼夜阶段对不同行为类型的影响的信息，并表明昼夜节律对学习和记忆的重要性。尽管我们没有检测到焦虑和空间学习/记忆方面的差异，但昼夜节律可能与其他因素相互作用影响这些行为。
