Aruffo A, Seed B
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
EMBO J. 1987 Nov;6(11):3313-6. doi: 10.1002/j.1460-2075.1987.tb02651.x.
The human CD7 antigen (gp40) is a cell surface glycoprotein found on thymocytes and mature T-cells. It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage, and the most reliable clinical marker of T-cell acute lymphocytic leukemia. This report describes the isolation and nucleotide sequence of a full length CD7 cDNA, and of a cDNA for an unusual intron-bearing precursor. The DNA sequence of the clone predicts a highly glycosylated membrane protein with homology to members of the immunoglobulin superfamily, and no relationship to known oncogenes. Over-expression of CD7 RNA was observed in only one T-cell tumor line, and genomic DNA rearrangement was not observed in any lines. Prompted by a recent suggestion that CD7 plays a role in IgM binding, COS cells expressing CD7 were tested and found not to bind IgM or IgM immune complexes.
人类CD7抗原（gp40）是一种在胸腺细胞和成熟T细胞上发现的细胞表面糖蛋白。它是T淋巴细胞谱系细胞上最早出现的抗原之一，也是T细胞急性淋巴细胞白血病最可靠的临床标志物。本报告描述了全长CD7 cDNA以及一个含有不寻常内含子的前体cDNA的分离和核苷酸序列。该克隆的DNA序列预测出一种高度糖基化的膜蛋白，与免疫球蛋白超家族成员具有同源性，与已知癌基因无关。仅在一个T细胞肿瘤系中观察到CD7 RNA的过表达，在任何系中均未观察到基因组DNA重排。最近有人提出CD7在IgM结合中起作用，受此启发，对表达CD7的COS细胞进行了测试，发现它们不结合IgM或IgM免疫复合物。
