CD7蛋白在肿瘤的T细胞浸润中起着关键作用。

CD7 protein plays a crucial role in T cell infiltration in tumors.

作者信息

Sheng Binjie, Zhang Kailu, Tian Shuaiyu, Ma Renyuxue, Li Zixuan, Wu Hai, Wang Tian, Jiang Licui, You Fengtao, An Gangli, Meng Huimin, Yang Lin, Liu Xin

机构信息

Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.

PersonGen BioTherapeutics (Suzhou) Co., Ltd., PR China.

出版信息

Heliyon. 2023 Jun 15;9(6):e16961. doi: 10.1016/j.heliyon.2023.e16961. eCollection 2023 Jun.

DOI:10.1016/j.heliyon.2023.e16961

PMID:37416646

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10320036/

Abstract

CD7 protein as a target is being used to treat CD7 lymphoma; however, the role of CD7 in the hematopoietic system remains largely unknown. Therefore, we evaluated the effects of CD7 KO in mice. The differentiation of the hematopoietic system in the bone marrow and the number of various cell types in the thymus and spleen did not differ between CD7 KO and WT mice. After subcutaneous inoculation of B16-F10 melanoma cells, tumors from CD7 KO mice grew more rapidly, and the proportion of CD8 T cells in the spleen and tumors decreased. In vitro, the infiltration and adhesion of CD8 T cells from the spleen of CD7 KO mice were weakened. Blocking CD7 in normal T cells did not alter the migration and infiltration, but in Jurkat, CCRF-CEM, and KG-1a tumor cell lines, migration and invasion were significantly reduced after blocking CD7. Therefore, CD7 does not affect hematopoietic system development but plays a crucial role in T cell infiltration into tumors.

摘要

CD7蛋白作为靶点正被用于治疗CD7淋巴瘤；然而，CD7在造血系统中的作用仍 largely未知。因此，我们评估了CD7基因敲除小鼠的效应。CD7基因敲除小鼠与野生型小鼠相比，骨髓中造血系统的分化以及胸腺和脾脏中各种细胞类型的数量并无差异。皮下接种B16 - F10黑色素瘤细胞后，CD7基因敲除小鼠的肿瘤生长更快，脾脏和肿瘤中CD8 T细胞的比例降低。在体外，CD7基因敲除小鼠脾脏中CD8 T细胞的浸润和黏附减弱。在正常T细胞中阻断CD7并未改变其迁移和浸润，但在Jurkat、CCRF - CEM和KG - 1a肿瘤细胞系中，阻断CD7后迁移和侵袭显著降低。因此，CD7不影响造血系统发育，但在T细胞浸润肿瘤中起关键作用。