Leitner Judith, Reutner Katharina, Essler Sabine E, Popow Irene, Gerner Wilhelm, Steinberger Peter, Saalmüller Armin
Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Vet Immunol Immunopathol. 2012 Mar 15;146(1):27-34. doi: 10.1016/j.vetimm.2012.01.012. Epub 2012 Jan 21.
During the last decades for several species--e.g. swine--many mAb to leukocyte-specific molecules have been developed and clusters of differentiation corresponding to human CD could be established. However, for a significant amount of the raised mAb the corresponding antigens were not characterized on the molecular level and therefore preliminary clusters--in swine so-called Swine workshop clusters (SWC)--were established. These clusters contain antigens with currently no obvious orthologs to human leukocyte differentiation antigens. In this study, we describe the generation of a eukaryotic cDNA expression library from in vitro activated porcine peripheral blood mononuclear cells. Screening of this library with an antibody recognizing SWC1 enabled isolation and sequencing of cDNAs coding for the porcine SWC1 molecule. A BLAST search of the obtained sequence revealed that SWC1 is the orthologous molecule of human CD52. Therefore, our study provides the basis for comparative studies on the role of CD52 in different mammalian species. In addition, the established cDNA library can be used for investigation of additional SWC-defined molecules.
在过去几十年中，针对几种物种——例如猪——已经开发出了许多针对白细胞特异性分子的单克隆抗体（mAb），并且可以建立与人类分化簇（CD）相对应的猪的分化簇。然而，对于大量产生的单克隆抗体，其相应抗原在分子水平上尚未得到表征，因此建立了初步的分化簇——在猪中称为猪研讨会分化簇（SWC）。这些分化簇包含目前与人类白细胞分化抗原没有明显直系同源物的抗原。在本研究中，我们描述了从体外活化的猪外周血单个核细胞生成真核cDNA表达文库的过程。用识别SWC1的抗体筛选该文库，使得能够分离和测序编码猪SWC1分子的cDNA。对获得的序列进行BLAST搜索发现，SWC1是人类CD52的直系同源分子。因此，我们的研究为比较研究CD52在不同哺乳动物物种中的作用提供了基础。此外，建立的cDNA文库可用于研究其他SWC定义的分子。
