Lim Jun-Hyung, Nam Sang Hwan, Kim Jongwoo, Kim Nam Hoon, Park Gun-Soo, Maeng Jin-Soo, Yook Se-Jin
School of Mechanical Engineering, Hanyang University, Seoul 04763, South Korea.
Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Yuseong, Daejeon 34114, South Korea.
J Biomech Eng. 2022 Jul 1;144(7). doi: 10.1115/1.4053504.
In this study, a three-stage bio-aerosol sampler with a sampling flow rate of 170 L/min was designed and fabricated for sampling the bio-aerosols released during human breathing and coughing, and its performance was evaluated. The sampler was constructed using a cyclone separator with a cutoff size of 2.5 μm as a preseparator, a multinozzle virtual impactor with a cutoff size of 0.34 μm as an aerosol concentrator, and a Bio-Sampler, which is a commercial product, for collecting bio-aerosols in a collection fluid. The collection efficiency of the sampler was evaluated through simulations and experiments. Only particles with sizes of 0.1-4 μm were selectively collected in the collection fluid. Bacteriophage bio-aerosols were sampled using the developed sampler and ACD-200 Bobcat sampler, which is a commercial product. The amounts of collected bacteriophages were compared using the polymerase chain reaction (PCR) technique. The sampling performance of the developed sampler was similar to that of the ACD-200 Bobcat sampler. Moreover, the developed sampler showed its ability to sample bio-aerosols of a specific size range and collect them directly in a collection fluid for the PCR analysis. Therefore, the developed sampler is expected to be useful for indoor environmental monitoring by effectively sampling the bio-aerosols released indoors during human breathing and coughing.
在本研究中，设计并制造了一种采样流速为170升/分钟的三级生物气溶胶采样器，用于采集人类呼吸和咳嗽过程中释放的生物气溶胶，并对其性能进行了评估。该采样器采用一个截止尺寸为2.5微米的旋风分离器作为预分离器、一个截止尺寸为0.34微米的多喷嘴虚拟撞击器作为气溶胶浓缩器以及一个Bio-Sampler（一种商业产品）来在收集液中收集生物气溶胶。通过模拟和实验对该采样器的收集效率进行了评估。只有尺寸为0.1 - 4微米的颗粒被选择性地收集在收集液中。使用所开发的采样器和一种商业产品ACD - 200 Bobcat采样器对噬菌体生物气溶胶进行了采样。使用聚合酶链反应（PCR）技术比较了收集到的噬菌体数量。所开发采样器的采样性能与ACD - 200 Bobcat采样器相似。此外，所开发的采样器显示出能够对特定尺寸范围的生物气溶胶进行采样，并将它们直接收集在收集液中用于PCR分析的能力。因此，预计所开发的采样器通过有效采集人类呼吸和咳嗽过程中在室内释放的生物气溶胶，将对室内环境监测有用。
