College of Chemistry and Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China.
Key Laboratory of Chemical Additives for China National Light Industry, Xi'an, China.
Mikrochim Acta. 2022 Jan 11;189(2):58. doi: 10.1007/s00604-021-05085-5.
Immunochromatography testing strips (ICTs) promise to become the point-of-care test format for early diagnosis due to their convenience, low cost, and simplification. However, the insufficient signal intensity and limited sensitivity of this format hamper their application. Herein, we overcame these limitations by integrating rod-like ferric oxyhydroxide (β-FeOOH) nanoparticles with ICTs. By varying the concentration of PEI, a one-pot, mild-temperature hydrolysis method was adapted for the synthesis and morphology regulation of β-FeOOH nanorod. Due to the excellent enzyme-like catalytic activity toward peroxidase substrates (TMB) in the presence of hydrogen peroxide (HO), the β-FeOOH nanorod in ICTs served as a signal generator and the nanozyme for signal amplification. The proof-of-concept work was performed for the detection of human chorionic gonadotropin (hCG). A two fold improvement of detection sensitivity was achieved compared to the sensitivity of conventional Au NPs-based ICTs. These results show that β-FeOOH-based ICT has a potential application in POCT detection in clinical diagnostics.
免疫层析检测条（ICTs）因其方便、低成本和简化而有望成为即时检测的检测形式，用于早期诊断。然而，这种格式的信号强度不足和灵敏度有限限制了它们的应用。在此，我们通过将棒状的氢氧化铁纳米粒子（β-FeOOH）与 ICTs 结合来克服这些限制。通过改变 PEI 的浓度，采用一锅温和温度水解法来合成和调节β-FeOOH 纳米棒的形态。由于在存在过氧化氢（HO）的情况下对过氧化物酶底物（TMB）具有优异的类酶催化活性，ICTs 中的β-FeOOH 纳米棒可用作信号发生器和纳米酶来进行信号放大。该工作已用于人绒毛膜促性腺激素（hCG）的检测。与传统的基于金纳米粒子的 ICTs 的灵敏度相比，检测灵敏度提高了两倍。这些结果表明，基于β-FeOOH 的 ICT 在临床诊断中的 POCT 检测中具有潜在的应用。
