白细胞介素-3的产生作为小鼠T淋巴细胞活化的敏感指标。
Interleukin-3 production as a sensitive measure of T-lymphocyte activation in the mouse.
作者信息
Dotsika E N, Sanderson C J
机构信息
National Institute for Medical Research, The Ridgeway, Mill Hill, London, U.K.
出版信息
Immunology. 1987 Dec;62(4):665-8.
Abstract
Interleukin-3 activity is produced by T cells at an early phase after antigenic stimulation, and its quantitation provides a sensitive and non-destructive method for assaying T-cell activation. It can be detected before increased uptake of [3H]thymidine into DNA occurs, and is more sensitive than measuring [3H]thymidine uptake for low levels of T-cell activation. This method has been shown to be useful in measuring activation of T-cell clones and in detecting antigens that have been blotted onto nitrocellulose.
摘要
白细胞介素-3活性是在抗原刺激后的早期由T细胞产生的，其定量为检测T细胞活化提供了一种灵敏且非破坏性的方法。在[3H]胸腺嘧啶核苷摄入DNA增加之前就能检测到它，并且对于低水平的T细胞活化，它比测量[3H]胸腺嘧啶核苷摄入更灵敏。该方法已被证明在测量T细胞克隆的活化以及检测已印迹到硝酸纤维素上的抗原方面是有用的。
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1
Interleukin 2 production by alloantigen (H-2) activated T cells.
Aust J Exp Biol Med Sci. 1981 Aug;59(4):413-26. doi: 10.1038/icb.1981.35.
2
Differential requirements for antigen processing by macrophages for lysozyme-specific T cell hybridomas.巨噬细胞对溶菌酶特异性T细胞杂交瘤进行抗原加工的不同要求。
J Immunol. 1984 Mar;132(3):1077-9.
3
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.用于细胞生长和存活的快速比色测定法：应用于增殖和细胞毒性测定。
J Immunol Methods. 1983 Dec 16;65(1-2):55-63. doi: 10.1016/0022-1759(83)90303-4.
4
Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.永久性因子依赖性多能（红系/中性粒细胞/嗜碱性粒细胞）造血祖细胞系的证明。
Proc Natl Acad Sci U S A. 1983 May;80(10):2931-5. doi: 10.1073/pnas.80.10.2931.
5
6
Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens.通过内源性酶己糖胺酶测量细胞数量。在检测淋巴因子和细胞表面抗原中的应用。
J Immunol Methods. 1984 Mar 16;67(2):379-88. doi: 10.1016/0022-1759(84)90477-0.
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Purification of virus glycoproteins by affinity chromatography using Lens culinaris phytohaemagglutinin.利用菜豆植物血凝素通过亲和层析法纯化病毒糖蛋白。
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