Greenberger J S, Sakakeeny M A, Humphries R K, Eaves C J, Eckner R J
Proc Natl Acad Sci U S A. 1983 May;80(10):2931-5. doi: 10.1073/pnas.80.10.2931.
Multipotential hematopoietic progenitor cell lines have been established from nonadherent cell populations removed from continuous mouse bone marrow cultures. Clonal sublines of lines B6SUtA or B6JUt derived from single cells formed mixed colonies containing erythroid cells, neutrophil-granulocytes, and basophil/mast cells in semisolid medium containing erythropoietin and conditioned medium from pokeweed mitogen-stimulated spleen cells. Each of several subclones of cell line Ro cl formed colonies containing eosinophils, neutrophil-granulocytes, and basophil/mast cells in semisolid medium. Multipotentiality was maintained in vitro for over 2 1/2 years. In contrast, cell line 32D formed basophil/mast cell colonies with no detectable differentiation to other pathways. Multipotential cell lines did not produce detectable spleen colonies (CFUs) in vivo, nor did intravenous inoculation of up to 5 X 10(7) cells protect lethally irradiated mice from bone marrow failure. Newborn and adult mice inoculated with 5 X 10(7) cells showed no detectable leukemia or solid tumors after one year. Both multipotential and committed basophil/mast cell lines demonstrated absolute dependence upon a source of a growth factor(s) found in medium conditioned by WEHI-3 cells. These cell lines should be of value in studies of the regulation of hematopoietic stem cell differentiation in vitro.
多能造血祖细胞系已从连续小鼠骨髓培养物中去除的非贴壁细胞群体中建立。源自单细胞的B6SUtA或B6JUt系的克隆亚系在含有促红细胞生成素和商陆有丝分裂原刺激的脾细胞条件培养基的半固体培养基中形成了含有红细胞、中性粒细胞和嗜碱性粒细胞/肥大细胞的混合集落。细胞系Ro cl的几个亚克隆中的每一个在半固体培养基中形成了含有嗜酸性粒细胞、中性粒细胞和嗜碱性粒细胞/肥大细胞的集落。多能性在体外维持了超过2年半。相比之下，细胞系32D形成了嗜碱性粒细胞/肥大细胞集落，未检测到向其他途径的分化。多能细胞系在体内未产生可检测到的脾集落（CFUs），静脉注射多达5×10⁷个细胞也不能保护受致死性照射的小鼠免于骨髓衰竭。接种5×10⁷个细胞的新生小鼠和成年小鼠在一年后未显示可检测到的白血病或实体瘤。多能和定向嗜碱性粒细胞/肥大细胞系都表现出对WEHI-3细胞条件培养基中发现的生长因子来源的绝对依赖性。这些细胞系在体外造血干细胞分化调控研究中应具有价值。
