Department of Experimental Pathology, Ningbo Clinical Pathology Diagnosis Center, Ningbo, China.
Institute of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, Ningbo, China.
J Clin Lab Anal. 2022 Feb;36(2):e24192. doi: 10.1002/jcla.24192. Epub 2022 Jan 11.
Long noncoding RNAs (lncRNAs) play important roles in almost every stage of cancer development. Given the competing endogenous RNA (ceRNA) hypothesis for the regulation of gene expression, we investigated the role of LINC00152 as a ceRNA in gastric cancer (GC) cells.
Gastric cancer cell lines were used in this study. Mimics of miRNAs and siRNA were used to evaluate the interaction between LINC00152 and HMGA1. The quantitative real-time polymerase chain reaction was performed for analyzing gene expression at the transcriptional level. Flow cytometry assay of cell cycle and western blot analysis of related protein expression levels were performed. Online databases such as TCGA and TIMER were used to determine the possibility of HMGA1 and LINC00152 as GC markers and their role in immune infiltration.
Treating GC cell lines with LINC00152 siRNAs downregulated the expression of HMGA1. The cell cycle was arrested in the S phase following a reduction in LINC00152 or HMGA1 expression, whereas the expression of the cell cycle inhibitor P27 increased. In this study, we showed that acting as a ceRNA of HMGA1, LINC00152 has the same function as HMGA1, considering that it could control the cell cycle and promote GC cell proliferation. The TCGA database showed that LINC00152 might be used as a diagnostic marker for GC.
These findings provide mechanistic insights into the role of LINC00152 as a ceRNA to regulate HMGA1 expression in GC cells, where it can promote the proliferation of the GC cells by regulating the expression of the P27.
长链非编码 RNA（lncRNAs）在癌症发展的几乎每个阶段都发挥着重要作用。鉴于竞争内源性 RNA（ceRNA）假说可调节基因表达，我们研究了 LINC00152 作为胃癌（GC）细胞 ceRNA 的作用。
本研究使用了胃癌细胞系。使用 miRNA 的模拟物和 siRNA 来评估 LINC00152 和 HMGA1 之间的相互作用。采用定量实时聚合酶链反应（qRT-PCR）在转录水平上分析基因表达。通过细胞周期流式细胞术分析和相关蛋白表达水平的 Western blot 分析来进行实验。使用 TCGA 和 TIMER 等在线数据库来确定 HMGA1 和 LINC00152 作为 GC 标志物的可能性及其在免疫浸润中的作用。
用 LINC00152 siRNAs 处理 GC 细胞系可下调 HMGA1 的表达。降低 LINC00152 或 HMGA1 的表达会导致细胞周期停滞在 S 期，而细胞周期抑制剂 P27 的表达增加。在这项研究中，我们表明，作为 HMGA1 的 ceRNA，LINC00152 具有与 HMGA1 相同的功能，因为它可以控制细胞周期并促进 GC 细胞增殖。TCGA 数据库表明，LINC00152 可能作为 GC 的诊断标志物。
这些发现为 LINC00152 作为 ceRNA 调节 GC 细胞中 HMGA1 表达的作用提供了机制见解，它可以通过调节 P27 的表达来促进 GC 细胞的增殖。
