State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, China.
J Cell Biochem. 2019 Mar;120(3):3747-3756. doi: 10.1002/jcb.27655. Epub 2018 Oct 10.
LINC00152 has been considered to be associated with the tumorigenesis and the occurrence of gastric cancer; however, the mechanism of LINC00152 has yet to be fully elucidated. In the present study, the expression levels of LINC00152 in tissues, serum, and peripheral blood mononuclear cells (PBMCs) of patients with gastric cancer were determined using real-time polymerase chain reaction. The functions of LINC00152 with respect to the proliferation, apoptosis, migration, and invasive abilities of the gastric cancer cells were evaluated by cell proliferation analysis, flow cytometry, cell scratch wound assay, and transwell migration experiments. A mouse xenotransplant model of gastric tumors was established to detect the role of LINC00152 in vivo, and the expression levels of B-cell lymphoma-2 (Bcl-2) family proteins were investigated by Western blot analysis. The results revealed that LINC00152 was overexpressed in tissues, serum, and PBMCs of patients with gastric cancer. Moreover, LINC00152 could promote the migration and invasive abilities and suppress the apoptosis, of gastric cancer cells through regulating the Bcl-2 protein family. LINC00152 could bind with Bcl-2 directly to induce the activation of cell cycle signaling, and this may be a potential target for the therapy of gastric cancer in the future.
LINC00152 被认为与胃癌的发生和发生有关;然而，LINC00152 的机制尚未完全阐明。在本研究中，通过实时聚合酶链反应测定了胃癌患者组织、血清和外周血单个核细胞(PBMCs)中 LINC00152 的表达水平。通过细胞增殖分析、流式细胞术、细胞划痕实验和 Transwell 迁移实验评估了 LINC00152 对胃癌细胞增殖、凋亡、迁移和侵袭能力的影响。建立了小鼠胃癌移植瘤模型，检测 LINC00152 在体内的作用，并通过 Western blot 分析研究了 B 细胞淋巴瘤-2(Bcl-2)家族蛋白的表达水平。结果表明，LINC00152 在胃癌患者的组织、血清和 PBMCs 中过度表达。此外，LINC00152 通过调节 Bcl-2 蛋白家族促进胃癌细胞的迁移和侵袭能力，并抑制其凋亡。LINC00152 可以直接与 Bcl-2 结合，诱导细胞周期信号的激活，这可能是未来胃癌治疗的一个潜在靶点。
