Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China.
Chempluschem. 2022 Jan;87(1):e202100478. doi: 10.1002/cplu.202100478.
DNA origami templates are known to exhibit many advantages to integrate functional components at desirable locations for nanoelectronic applications. In order to immobilize conducting or semiconducting species in a bottom-up approach, the programmed assembly of DNA templates is of utmost necessity. This report demonstrates the silver nanowires enabled bridging of two linear DNA origami (DO) nanostructures by utilizing the host-guest interaction of biotin-STV and sequence-specific silver metallization of poly(dG-dC) DNA nanowires (in 10 % yield) using (dA) coated AgNPs (15 nm). The enzymatic synthesis of 750 bp, 1500 bp and 3000 bp bis-biotinylated poly(dG-dC), facile synthesis of 1 : 1 biotin-STV and silver-nanowire bridged DNA templates were characterized by gel electrophoresis, atomic force microscope imaging techniques. The strategy utilized here provides a method that can precisely connect heterogeneous templates towards bottom-up fabrication of practical nanoelectronics.
DNA 折纸模板被公认为在纳米电子应用中具有将功能组件集成到理想位置的许多优势。为了在自下而上的方法中固定导电或半导体物质，对 DNA 模板进行程序化组装是极其必要的。本报告展示了通过利用生物素-STV 的主-客体相互作用以及聚(dG-dC) DNA 纳米线的序列特异性银金属化（在 10%的收率下），使用（dA）涂覆的 AgNPs（15nm），银纳米线使两个线性 DNA 折纸（DO）纳米结构桥接。通过凝胶电泳、原子力显微镜成像技术对 750bp、1500bp 和 3000bp 的双生物素化聚(dG-dC)、1:1 生物素-STV 和银纳米线桥接 DNA 模板的简便合成进行了表征。这里利用的策略提供了一种可以精确连接异质模板的方法，从而朝着实用纳米电子学的自下而上制造迈进。
