Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine and State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200120, P. R. China.
Small. 2021 Nov;17(47):e2103877. doi: 10.1002/smll.202103877. Epub 2021 Oct 11.
DNA origami has been widely used as a modular platform for condensation of functional molecules to assemble optical, electronic, and biological components. However, the heterogeneous condensation with greater diversities in chemical composition templated with DNA origami is still challenging. Herein, a programmable deposition method is developed to precisely condense silver-silica nanohybrids on DNA origami templates. First, the site-specific metallization of Ag is achieved by thiol group-initiated silver reduction at the designed areas of DNA origami. Next, cysteamine is used to selectively modify the condensed Ag surface with positively charged amino groups for creating an electronically different environment for site-specific placement of silica by a modified Stöber method. Using these strategies, customized patterning of both silver and silica on tubular and rectangular DNA origami nanostructures is successfully achieved with nanoscale spatial resolution. These findings will greatly facilitate the development of DNA nanotechnology-based bottom-up nanofabrication.
DNA 折纸已被广泛用作功能分子凝聚的模块化平台，以组装光学、电子和生物组件。然而，利用 DNA 折纸模板进行具有更大化学成分多样性的异质凝聚仍然具有挑战性。在此，开发了一种可编程沉积方法，以在 DNA 折纸模板上精确地凝聚银-二氧化硅纳米杂化体。首先，通过在 DNA 折纸的设计区域进行巯基引发的银还原，实现 Ag 的位点特异性金属化。接下来，半胱胺用于选择性修饰凝聚的 Ag 表面，引入带正电荷的氨基，通过改良的 Stöber 方法在特定位置上形成具有不同电子环境的二氧化硅。利用这些策略，成功地在管状和矩形 DNA 折纸纳米结构上实现了银和二氧化硅的定制图案化，具有纳米级空间分辨率。这些发现将极大地促进基于 DNA 纳米技术的自下而上纳米制造的发展。
