Department of Chemical Engineering, Tecnologico de Monterrey, Monterrey, Nuevo León 64849, México.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
ACS Appl Bio Mater. 2021 Jan 18;4(1):392-398. doi: 10.1021/acsabm.0c01474. Epub 2021 Jan 5.
Whatman No. 1 chromatography paper is widely used as a substrate for cellulose-based immunoassays. The immobilized proteins are used to capture target biomarkers for detection. However, alternative paper substrates may facilitate mass production of immunoassays as diagnostic tests. Here, we assessed the physical characteristics and protein immobilization capabilities of different commercial papers. Some substrates fulfilled our design criteria, including adequate flow rate and sufficient protein immobilization for efficient target capture. This study demonstrates that a variety of paper substrates can be bioactivated and used to capture target biomarkers, enabling development of affordable diagnostic tests from a range of starting materials.
沃特曼 No.1 色谱纸被广泛用作基于纤维素的免疫分析的底物。固定化蛋白质用于捕获用于检测的靶生物标志物。然而，替代的纸基可能有利于免疫分析的大规模生产作为诊断测试。在这里，我们评估了不同商业纸张的物理特性和蛋白质固定化能力。一些底物满足了我们的设计标准，包括足够的流速和足够的蛋白质固定化以实现高效的靶捕获。本研究表明，各种纸基可以被生物激活并用于捕获靶生物标志物，从而能够从各种起始材料开发出负担得起的诊断测试。
